Summary
A three-dimensional (3D) graphic model of a single-chain Fv (scFv) which was derived from an anti-human placental acidic isoferritin (PAF) monoclonal antibody (MAb) was constructed by a homologous protein-predicting computer algorithm on Silicon graphic computer station. The structure, surface static electricity and hydrophobicity of scFv were investigated. Computer graphic modelling indicated that all regions of scFv including the linker, variable regions of the heavy (VH) and light (VL) chains were suitable. The VH region and the VL region were involved in composing the “hydrophobic pocket”. The linker was drifted away VH and VL regions. The complementarity determining regions (CDRs) of VH and VL regions surrounded the “hydrophobic pocket”. This study provides a theory basis for improving antibody affinity, investigating antibody structure and analyzing the functions of VH and VL regions in antibody activity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arosio P, Adelman T G, Drysdale J W. On ferritin heterogeneity: further evidence for heteropolymers. J Biol Chem, 1978, 253: 4451
Powell L, Alpert E, Isselbacher K Jet al. Human iosferritins: Organ specific iron and apoferritin distribution. Brit J Haematol, 1975, 30: 47
Dinari G, Zahavi I, Marcus Het al. Placental ferritin in coeliac disease: relation to clinical stage, origin, and possible role in the pathogenesis of malignancy. Gut, 1991, 32: 999
Rosen A, Hafner E, Auerbach Let al. Placental isoferritin in pregnancies with small-for-gestational age fetuses. Prenat Diagn, 1996, 16: 641
Shterman N, Kupfer B, Moroz C. Comparison of transferrin receptors, iron content and isoferritin profile in normal and malignant human breast cell lines. Pathobiology, 1991, 59: 19
Rosen H R, Flex D, Sierer Met al. Monoclonal antibody CM-H-9 detects circulating placental isoferritin in the serum of patients with visceral metastases of breast cancer. Cancer Lett, 1991, 59: 145
Zhou C, Shao J, Shen Get al. cDNA sequence analysis of monoclonal antibodies against the human placental acidic isoferritin. Hybridoma, 1998, 17 (3): 275
Whitlow M, Filpula D. Single-chain Fv proteins and their fusion proteins. Methods Enzymol, 1991, A2: 97
Pearson W R, Lipman D J. Improved tools for biological sequence comparison. Proc Natl Acad Sci USA, 1988, 85: 2444
Eck M J, Sprang S R. The structure of tumor necrosis factor-alpha at 2. 6 Aresolution I mpliations for receptor binding. J Biol Chem, 1989, 264: 17595
Jones E Y, Stuart D I, Walker N P. Structure of tumor necrosis factor. Nature, 1989, 338: 225
Lüthy R, Bowie J U, Eisenberg D. Assessment of protein models with three-dimensional profiles. Nature, 1992, 356: 83
Sander C, Vriend G, Bazan Fet al. Protein design on computers. Five new proteins: Shpilka, Grender, Fingerclasp, Leather, and Aida. Protein, 1992, 12(2): 105
Bhat T N, Bentley G A, Fischmann T Oet al. Small rearrangement in structures of Fv and Fab fragments of antibody D1. 3 on antigen binding. Nature, 1990: 347 (6292): 48
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chun, Z., Guanxin, S., Huifen, Z. et al. Construction and analysis of three-dimensional graphic model of single-chain Fv derived from an anti-human placental acidic isoferritin monoclonal antibody by computer. Current Medical Science 20, 23–25 (2000). https://doi.org/10.1007/BF02887667
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02887667