Skip to main content
SpringerLink
Log in

Studies on the glutamylpeptide antigen (GPP) in the genusBacillus

The frequence of its occurrence and presence in the cell wall

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The frequency of occurrence of the glutamylpeptide (GPP) antigen in genusBacillus is described. The presence of this antigen was detected in 77.8% of strains using immunochemical assay, and in 100% when the immunofluorescent method was used. GPP appears to be a component of the cell wall and its presence is not bound to a morphologically demonstrable capsule. In addition, it was shown that in some strains possessing the myxomatose type of growth, the capsular GPP may separatein toto from the body of the cell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Avakyan, A. A., Katz, L. N., Levina, K. N., Pavlova, I. B.:Structure and composition of the Bacillus anthracis capsule. J. Bacteriol. 90: 1082, 1965.

    PubMed  CAS  Google Scholar 

  • Baillie, A.:Antigens of B. cereus: a comparison of a parent strain, an asporogenic variant and cell fractions. J. appl. Bact. 30: 230, 1967.

    CAS  Google Scholar 

  • Barber, C.:Critique des expériences tendant à mettre en évidence une “capsule” chez Bacillus anthracis. Ann. Inst. Pasteur 111: 226, 1966.

    CAS  Google Scholar 

  • Barber, C., Eustatziu, S., Zilisteanu, E.:Über die Lokalization der spezifischen Substanzen bei Klebsiella und beim Anthraxbacillus. Zbl. Bakt. (Orig.), 202: 67, 1967.

    CAS  Google Scholar 

  • Bovarnick, M.:The formation of extracellular d(−)glutamic acid polypeptide by Bacillus subtilis. J. biol. Chem. 145: 415, 1942.

    Google Scholar 

  • Dubos, R. J.:Factors affecting the yield of specific enzyme in cultures of bacillus decomposing the capsular polysaccharide of type III pneumococcus. J. exptl. Med. 55: 377, 1932.

    Article  CAS  Google Scholar 

  • Goodman, J. W., Nitecki, D. E.:Immunochemical studies on the poly-γ-d-glutamyl capsule of Bacillus anthracis. I. Characterization of the polypeptide and of the specificity of its reaction with rabbit antisera. Biochemistry 5: 657, 1966.

    Article  PubMed  CAS  Google Scholar 

  • Housewright, R. D.:The biosynthesis of homo polymeric peptides. In: The Bacteria, ed. G. I. C. Gunsalus and R. J. Stanier, vol. 3, p. 389. New York and London. Academic Press, 1962.

    Google Scholar 

  • Ivánovics, G.:Das Vorkommen der spezifischen Kapselsubstanz der Milzbrandbazillen in verschiedenen, aeroben, sporentragenden Saptophytenbazillen. Zblt. Bakt. (Orig.) 138: 211, 1937.

    Google Scholar 

  • Ivánovics, G.:The serological specificity of synthetic polymers of glutamic acid. (In Czech) Proceedings: Anthropozoonózy, Stát. zdrav. nakl. (Prague). p. 404. 1958.

  • Ivánovics, G., Bruckner, G.:Chemische und immunologische Studien über den Mechanismus der Milzbrandinfection und-Immunität. I. Die chemische Struktur der Kapselsubstanz des Milzbrandbazillus und der serologisch identischen spezifischen Substanz des Bazillus mezentericus. Z. Immun. Forsch. exper. Ther. 90: 304, 1937.

    Google Scholar 

  • Keppie, J., Harris-Smith, P. W., Smith, H.:The chemical basis of the virulence of Bacillus anthracis. IV. Its aggressins and their mode of action. Brit. J. exptl. Pathol. 44: 446, 1963.

    CAS  Google Scholar 

  • Kolesov, S. G., Michailov, N. A., Gorlov, G. V., Palgov, A. A.:Biological properties of vaccine STI and complicating factors at its application. (In Russian) Proceedings: Biopreparations, viruses and microbes. SELCHOZGIZ, vol. 6, p. 256, Moscow, 1956.

    Google Scholar 

  • Leonard, C. G., Thorne, C. B.:Studies on the nonspecific precipitation of basic serum proteins with gamma glutamyl polypeptides. J. Immunol. 87: 175, 1961.

    PubMed  CAS  Google Scholar 

  • Mastroeni, P., Nacci, A., Rocca, A.:Immunoelectric analysis of the antigenic components of Bacillus megatherium. J. Bacteriol. 94: 1073, 1967.

    Google Scholar 

  • Meynell, G. G., Lawn, A. M.:Inheritance of capsule and the manner of cell wall formation in Bacillus anthracis. J. gen. Microbiol. 39: 423, 1965.

    PubMed  CAS  Google Scholar 

  • Meynell, E., Meynell, G. G.:The roles of serum and carbondioxide in capsule formation by Bacillus anthracis. J. gen. Microbiol. 34: 153, 1964.

    PubMed  CAS  Google Scholar 

  • Meynell, G. G., Meynell, E.:The biosynthesis of poly-d-glutamic acid, the capsular material of Bacillus anthracis. J. gen. Microbiol. 43: 119, 1966.

    PubMed  CAS  Google Scholar 

  • Nordberg, B. K., Thorsell, W.:The effect of certain enzyme systems on the capsule of Bacillus anthracis. J. Bacteriol. 69: 367, 1955.

    PubMed  CAS  Google Scholar 

  • Salton, M. R. J.:The anatomy of the bacterial surface. Bacteriol. Rev. 25: 77, 1961.

    PubMed  CAS  Google Scholar 

  • Schneiderka, P., Vančuřík, J.:Studies on glutamylpeptide antigen in genus Bacillus. I. The preparation of d-glutamylpeptide by gel filtration. J. hyg. epid. microbiol. immunol. (Prague—in press.)

  • Škvařil, F., Rejnek, J.:Our experience with the microdification of immunoelectrophoresis. (In Czech) Czech. epid. microbiol. immunol. (Prague) 7: 414, 1958.

    Google Scholar 

  • Thorne, C. B.:Biochemical properties of virulent and avirulent strains of Bacillus anthracis. Ann. N. Y. Acad. Sci. 88: 1024, 1960.

    Article  PubMed  CAS  Google Scholar 

  • Thorne, C. B., Góméz, C. G., Housewright, R. D.:Synthesis of glutamic acid and glutamyl polypeptide by Bacillus anthracis. II. The effect of carbon dioxide on peptide production on solid media. J. Bacteriol. 63: 363, 1952.

    PubMed  CAS  Google Scholar 

  • Tomcsik, J., Guex-Holzer, S.:Anthrax-Polypeptid und andere Species-spezifische Substanzen der Kapsel in der Bazillus-Gruppe. Schweiz. Z. all. Path. Bakt. 14: 515, 1951.

    CAS  Google Scholar 

  • Torii, M.:Decapsulation of Bacillus anthracis. Med. J. Osaka Univ. 6: 725, 1955.

    CAS  Google Scholar 

  • Vančuřík, J.:Simple cultivation preparation of anthrax protective antigen and its serologic demonstration. (In Czech) Czech. epid. microbiol. immunol. (Prague) 19: 118, 1970.

    Google Scholar 

  • Utsumi, S., Torii, M., Kurimura, O., Yamamuro, H., Amano, T.:Immunochemical studies on bacterial glutamyl polypeptides. Biken's J., 2: 165, 1959.

    CAS  Google Scholar 

  • Yamamuro, H.:Studies on the entity of anti-polyglutamic acid antibody. Med. J. Osaka Univ. 16: 431, 1964.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Military Institute of Hygiene, Epidemiology and Microbiology, Prague 6

    J. Vančuřík & P. Schneiderka

Authors
  1. J. Vančuřík
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Schneiderka
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vančuřík, J., Schneiderka, P. Studies on the glutamylpeptide antigen (GPP) in the genusBacillus . Folia Microbiol 16, 74–82 (1971). https://doi.org/10.1007/BF02887474

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02887474

Keywords

Search

Navigation