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Establishment of Jurkat tet-on cell line

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Chinese Science Bulletin

Abstract

Tet-control system is developed to tightly control target gene expression in mammalian cells by using the regulatory elements of tetracycline-repressor of the transposor Tn10 fromE. coli. We have transfected reverse tetracycline-controlled transactivator gene (rtTA) into genome of Jurkat cells and established two Jurkat tet-on cell lines. Induction of luciferase reporter activity with doxycycline, a tetracycline derivative, is dose-dependent with a peak value of 32-fold increment. Establishment of Jurkat tet-on cell lines greatly facilitates quantitative studies on target gene functions in the cells.

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Authors and Affiliations

  1. State Key Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Institute of Basic Medical Sciences, Peking Union Medical College, 100005, Beijing, China

    Hongwei Wang, Jing Zhang, Ninghua Wu & Yufei Shen

Authors
  1. Hongwei Wang
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  2. Jing Zhang
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  3. Ninghua Wu
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  4. Yufei Shen
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Cite this article

Wang, H., Zhang, J., Wu, N. et al. Establishment of Jurkat tet-on cell line. Chin.Sci.Bull. 45, 532–536 (2000). https://doi.org/10.1007/BF02887100

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  • DOI: https://doi.org/10.1007/BF02887100

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