Skip to main content

Advertisement

SpringerLink
Log in

Heat-shock protein 90 inCandida albicans

  • Review
  • Published:
Chinese Science Bulletin

Abstract

Researches on Candidal heat-shock protein 90 (HSP90) in recent years are summarized.Candida albicans is a commensal pathogen in human and animals. In immunocompromised individuals it behaves as an opportunist pathogen, giving rise to superficial or systemic infections. Systemic candidosis is a common cause of death among immunocompromised and debilitated patients, in which the mortality is as high as 70%. HSP90 is now recognized as an immunodominant antigen inC. albicans and plays a key role in systemic candidosis as a molecular chaperone. The 47-ku peptide is the breakdown product of HSP90. Patients who has recovered from systemic candidosis produce high titre of antibodies to 47-ku antigen, whereas the fatal cases have little antibody or falling titres. The three commonest epitopes of candidal HSP90 have been mapped, epitopes C, B and H. Epitopes C and H are immunogenic. The antibody probes of both epitopes may be developed into a new serological test agents for systemic candidosis due to rather high specificity and sensitivity. The recent results establish HSP90 as an ATP-dependent chaperone that is involved in the folding of cell regulatory proteins and in the refolding of stress-denatured polypeptides. Some researches on fungal HSP90 and the treatment of patients with candidosis are reviewed as well.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. O’Connell, B. C., Xu, T., Walsh, T. J. et al., Transfer of a gene encoding the anticandidal protein histatin 3 to salivary glands, Hum. Gene. Ther., 1996. 18: 2255.

    Article  Google Scholar 

  2. Matthews, R. C.,Candida albicans HSP 90: link between protective and auto-immunity, J. Med. Microbiol., 1992, 36: 367.

    Article  PubMed  CAS  Google Scholar 

  3. Matthews, R. C., Burnie, J. P., The role of HSP90 in fungal infection, Immunol. Today, 1992, 13(9): 345.

    Article  PubMed  CAS  Google Scholar 

  4. Ellis, J., Proteins as molecular chaperones. Nature, 1987, 328: 378.

    Article  PubMed  CAS  Google Scholar 

  5. Walsh, P., Norris, D. A., Abe, J., Candida albicans induces selective expansion of human T lymphocytes expressing the T-cell receptor variable region V beta 5.1. J. Dermatol. Sci., 1996, 12(2): 140.

    Article  PubMed  CAS  Google Scholar 

  6. Matthews, R., Burnie, J. P., Lee, W., The application of epitope mapping in the development of a new serological test for systemic candidosis. J. Immunol. Methods, 1991, 143: 73.

    Article  PubMed  CAS  Google Scholar 

  7. Matthews, R., Burnie, J., Antibodies against Candida: potential therapeutics? Trends Microbiol., 1996, 4(9): 354.

    Article  PubMed  CAS  Google Scholar 

  8. Matthews, R., Hodgells, S., Burnie, J., Preliminary assessment of a human recombinant antibody fragment to HSP90 in murine invasive candidasis. J. Infect. Dis. 1995, 171(6): 1668.

    PubMed  CAS  Google Scholar 

  9. Matthews, R. C., Burnie, J. P., Howat, D. et al., Autoantibody to heat shock protein 90 can mediate protection against systemic candidosis, Immunology. 1991, 74: 20.

    PubMed  CAS  Google Scholar 

  10. Zhi-zhen, W., The new-peptide chain’s folding and chaperones, in The Advanced Field of Life Sciences Forward 21th Century (in Chinese) (ed. Li, B. J.). Guangdong: Guangzhou Science and Technology Press, 1996, 93.

    Google Scholar 

  11. Obermann, W. M. J., Sondermann, H., Russo, A. A. et al.,In vivo function of HSP90 is dependent on ATP binding and ATP hydrolysis. J. Cell Biol., 1998, 143(4): 901.

    Article  PubMed  CAS  Google Scholar 

  12. Schwartz, J. A., Mizukami, H., A metal-linked gapped zipper model is proposed for the 90KUa heat shock protein-estrogen receptor interface, Med Hypoth., 1991, 35: 140.

    Article  CAS  Google Scholar 

  13. Rebbe, N. F., Ware, J., Bertina, R. M. el al., Nucleotide sequence of a cDNA for a member of the human 90-kua heat-shock protein family. Gene, 1987. 53: 235.

    Article  PubMed  CAS  Google Scholar 

  14. Swoboda, R. K., Bertram, G., Budge, S. et al., Structure and regulation of the HSP90 gene from the pathogenic fungusCandida albicans, Infect. Immun., 1995, 63(11): 4506.

    PubMed  CAS  Google Scholar 

  15. Burnie, J. P., Matthews, R. C., Heal shock protein 88 and Aspergillus infection, J. Clin. Microbiol., 1991, 29(10): 2099.

    PubMed  CAS  Google Scholar 

  16. Hodgetts, S., Matthews, R., Morrissey, G. et al., Over-expression ofSaccharomyces cerevisiae HSP90 enhances the virulence of this yeast in mice, FEMS Immunol. Med. Microbiol., 1996, 16(3-4): 229.

    Article  PubMed  CAS  Google Scholar 

  17. Ziegler, E. J., Fisher, C. J., Sorung, C. L. el al., Treatment of gram-negative bacteria and septic shock with HA-IA human monoclonal antibody against endotoxin, New Engl. J. Med., 1991, 324: 429.

    PubMed  CAS  Google Scholar 

  18. Driscoll, J., Duan, C., Zuo, Y. et al., Candidacidal activity of human salivary histatin recombinant variants produced by site-directed mutagenesis. Gene, 1996, 177: 1–2, 29–34.

    Article  Google Scholar 

  19. Tsai, H., Bobek, L. A., Studies of the mechanism of human salivary histatin-5 candidacidal activity with histatin-5 variants and azole-sensitive and resistant Candida species, Antimicrob Agents Chemother, 1997, 41(10): 2224.

    PubMed  CAS  Google Scholar 

  20. Galea-Lauri, J., Richardson, A. J., Latchman, D. S. et al., Increased heat shock protein 90 (HSP90) expression leads to increased apoptosis in the monoblastoid cell line U937 following induction with TNF-alpha and cycioheximide: a possible role in immunopathology. J. Immunol., 1996, 157(9): 4109.

    PubMed  CAS  Google Scholar 

  21. Stahl, M., Ludwig, D., Fellermann, K. et al., Intestinal expression of human heat shock protein 90 in patients with Crohn’s disease and ulcerative colitis. Dig. Dis. Sci., 1998, 43(5): 1079.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China

    Li Wang & Xiaojuan Zhu

Authors
  1. Li Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaojuan Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Li Wang.

About this article

Cite this article

Wang, L., Zhu, X. Heat-shock protein 90 inCandida albicans . Chin.Sci.Bull. 45, 481–484 (2000). https://doi.org/10.1007/BF02887089

Download citation

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02887089

Keywords

Search

Navigation