Summary
To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with domestic immunomagnetic beads. The expression of the two adhesion molecules CDlla/CD18 and CD44 were examined. After separation, CD34 positive cells accounted for 51%–82% of the harvested cells and dye-resistance rate was 82%–88%. The expression of CD1WCD18 and CD44 on the surfaces of UB cells was 49.6%±10.2% and 37.7%±10.3% respectively. On BM cells they were 50.2%±6.2% and 34%±13.3% respectively. There were no significant differences in the expression of these two molecules. It was concluded that the cell separation method with domestic immunomagnetic beads was effective and the stem cells from UB could serve as an alternative source for transplantation.
Similar content being viewed by others
References
Ugelstad J, Soderberg L, Berge Aet al. Monodisperse polymer particles —a step forward for chromatography. Nature, 1983, 303:95
Hardingham J E, Kotasek D, Farmer Bet al. Immunobead PCR: A technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction. Can Res, 1993, 53:34, 553.
Uhlen M. Magnetic separation of DNA. Nature, 1989, 40:733
Enroth H, Engstrand L. Immunomagnetic separation and PCR for detection of Helicobacter Pylori in water and stool specimens. J Clin Microbiol, 1995, 33:2162
Long M W. Blood cell cytoadhension molecules. Exp Hematol, 1992, 20:228
Papayannopoulou T, Craddock C. Homing and trafficking of hematopoietic progenitor cells. Acta Hematol, 1997, 97:97
Reuss-Borst M A, Bullring H J, Klein Get al. Adhension molecules on CD34+ hematopoietic cells in normal human bone marrow and leukemia. Ann Hematol, 1992, 65:169
Reems J A, Mielcarek M, Torok-Storb B. Differential modulation of adhension markers with ex vivo expression of human umbilical CD34+ progenitor cells. Bone Marrow Transplant, 1997, 3:113
Watanabe T, Dare B, Heimann D Get al. GM-CSF mobilized peripheral blood CD34+ cells differ from steadystate bone marrow CD34+ cells in adhension molecule expression. Bone Marrow Transplant, 1997, 19:1175
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liangli, W., Ping, Z. & Yong, Y. Separation of CD34 positive cells and determinationof surface homing antigen. Current Medical Science 20, 132–133 (2000). https://doi.org/10.1007/BF02887051
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02887051