Summary
To study the protective effects of radix astragali against anoxic damages to in vitro cultured neurons in rats, NaCN was used to develop a hypoxic model of in vitro cultured neurons from newborn rat cerebral cortex. The cellular morphology, A value (cell survival number) and effluxes of lactate dehydrogenase(LDH) and K+ from cells were measured in the radix astragali group and the control group respectively. After 48 h of anoxia, A value was decreased from 0.325±0. 031 before anoxia to 0.145±0.011, the effluxes of LDH and K+ were increased from 65.80±2.90 U/L and 5. 23±0.11mmol/L before anoxia to 148. 80±8.40 U/L and 7.31±0.18 mmol/L., respectively. It was found that in the anoxic circumstance in the Radix astragali group, the mophological changes were mild, the effluxes of LDH and K+ were decreased and A value increased as compared with those in the control group. It was suggested that Radix astragali could protect the cultured rat neurons against anoxic damages in the anoxic circumstance.
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Xiaohua, H., Chengyan, L. & Shaozu, Y. Protective effects of radix astragali against anoxic damages to in vitro cultured neurons. Current Medical Science 20, 126–127 (2000). https://doi.org/10.1007/BF02887049
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DOI: https://doi.org/10.1007/BF02887049