Summary
A new kind of silane coupling agent, N-(β-aminoethyl)-γ-aminopropyl triethoxysilane, was used for DNA direct attachment on the surfaces of glass supports, then the immobilized DNA was hybridized with horseradish peroxidase (HRP)-labeled probe, and detected by using enhanced chemiluminescent method. In comparison with 7-aminopropyl triethoxysilane, the detection limits (S/N) of DNA were 10 pg and 75 pg respectively. Several experimental conditions of DNA attaching to glass supports were investigated, and the system of hybridization of nucleic acid on the surfaces of glass supports was developed.
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This project was supported by a grant from National Science Foundation of China (No. 39990570).
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Guojun, Z., Yikai, Z., Xiaoyan, W. et al. Covalent attachment of DNA to glass supports using a new silane coupling agent and chemiluminescent detection. Current Medical Science 20, 89–91 (2000). https://doi.org/10.1007/BF02887038
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DOI: https://doi.org/10.1007/BF02887038