Methodological study of cell separation with domestic immunomagnetic beads

Summary

To establish the method of cell separation with domestic immuomagnetic beads, three methods were investigated. Direct method, SPA method and Biotin-Avidin method were applied to separate cell strain Hut-78 and CD4 positive cells. Separation rate of strain Hut-78 was more than 90 % in direct method. Detachment rate with papain was over 95 %. Cell activity was well retained. SPA method and Biotin-Avidin methods were also effective, but the direct method was superior to the other two techniques. Before separated by the direct method, CD4 positive cells constituted 46.4 % ±6.4 % of mononuclear cells (MNC), but in eliminated suspension there was only 6.2 % ± 2.3 % CD4 positive cells left. In the separated part, 80.6 % ± 7.2 % of the cells combined with the beads. It is concluded that the direct method in separating cells had high sensitivity and specificity.