Summary
The availability of blood products for replacement therapy of hemophilia and allied disorders is still a formidable problem in most developing countries which consist of 80% of the world population. Fresh dried plasma (FDP) is a lyophilized form of fresh frozen plasma which can be stored at 4 °C, whereas fresh frozen plasma must be stored at −20 °C. A large-scale production of FDP from single units of fresh frozen plasma was achieved by the National Red Cross Blood Center in Thailand. This paper describes the method of preparation, biochemical and blood coagulation properties of FDP during one-year storage as well as its clinical usefulness. A batch of 179 bottles of FDP stored at 4 °C for one week, 3, 6, 9 and 12 months was studied in order to determine the biochemical and blood coagulation parameters. FDP has pH higher than that of fresh frozen plasma, but its value is within the safety range for infusion. Electrolyte and protein levels were comparable with those of fresh frozen plasma. Coagulant activities of factors I, II, V, VII, VIII, IX and X were about 10% lower than those of fresh frozen plasma; however, they are in the acceptable therapeutic range and all were stable during storage at 4 °C for up to one year. Over 4,000 bottles of FDP were used with good clinical and laboratory response and no serious side effects were observed in the treatment of bleeding in blood coagulation disorders and home care therapy of hemophilia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Denson K. W. E.: Assay of factor V. In:Biggs R. (Ed.): Human blood coagulation, haemostasis and thrombosis. 2nd ed., Blackwell Sci. Publ., Oxford, 1976; p. 702.
Ellis B. C., Stransky A.: A quick and accurate method for the determination of fibrinogen in plasma — J. Lab. clin. Med.58, 477, 1961.
Hardisty R. M., MacPherson J. C.: A one-stage factor VIII (antihemophilic globulin) assay and its use on venous and capillary plasma — Thrombos. Diathes. haemorrh. (Stuttg.)7, 215, 1962.
Lurie A., Oberwaldner B., Shapiro M.: Hemophilia home infusions with dried fresh plasma — S. Afr. med. J.49, 931, 1975.
Pechet L.: One-stage specific determination of factor II (prothrombin). In:Tocantins L. M., Kazal L. A. (Eds): Blood coagulation, hemorrhage and thrombosis. 2nd ed., Grune and Stratton, New York, 1964; p. 144.
Pechet L.: Determinations of factor VII-X. In:Tocantins L. M., Kazal L. A. (Eds): Blood coagulation, hemorrhage and thrombosis. 2nd ed., Grune and Stratton, New York, 1964; p. 213.
Pitney W. R.: Assay of factor IX. In:Dacie J. V., Lewis S. M. (Eds): Practical haematology. 5th ed., Churchill and Livingstone, London, 1975; p. 356.
Quick A. J.: The haemorrhagic disease and the physiology of haemostasis. C. C. Thomas, Springfield, 1942.
Wallace J.: Blood transfusion for clinicians. Churchill and Livingstone, London, 1977.
Author information
Authors and Affiliations
Additional information
This work was supported by a grant from the World Federation of Hemophilia and by Ramathibodi Research Grant Project 2525/1.
Rights and permissions
About this article
Cite this article
Isarangkura, P.B., Pundhawong, S., Pintadit, P. et al. Fresh dried plasma: A solution for the shortage of blood products in developing countries. La Ricerca Clin. Lab. 17, 349–354 (1987). https://doi.org/10.1007/BF02886918
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02886918