Summary
In the present paper we have observed the effect of discontinuous gradient two-step Percoll centrifugation on depleting mature T lymphocytes from normal bone marrow. The pre-/post-Percoll percentage of CD34+, and Leu4+ cells in MNC was counted by using APAAP technique. As the two-step Percoll centrifugation is simple, and time saving, and decreases the incidence of contamination of cultured cells as well, This technique may be of value in serum-free culture of hematopoietic cells and immunological studies.
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ji-sen, T., Bian-ming, W. An effective method for depleting mature T lymphocytes from bone marrow cells —two-step percoll centrifugation. J. Huazhong Univ. Sci. Technol. Med. Sci. 14, 128 (1994). https://doi.org/10.1007/BF02886790
Issue Date:
DOI: https://doi.org/10.1007/BF02886790