Summary
Seven alloantisera were selected showing the following characteristics: after platelet absorption they were positive with PHA-activated cells and negative with resting T and B lymphocytes from the same donors; this reactivity could be blocked by pretreatment of cells with a turkey anti-human β2-microglobulin serum and was not completely removed by further absorption with high doses of pooled platelets or with T or B lymphocytes from the positive donors; the reactivity of some of these antisera correlated with HLA-A antigens in a panel of PHA-activated cells and segregated with HLA haplotypes in families. It is still to be proven if the HLA determinants recognized on PHA-activated cells by these antisera are new epitopes of A,B,C molecules appearing upon activation or if they are carried by products of a new polymorphic locus linked to the HLA system.
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This work was supported byMinistero della Pubblica Istruzione (60%) and byRegione Piemonte, Ricerca Finalizzata № 36.
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Crepaldi, T., Amoroso, A., Curtoni, E.S. et al. Alloantisera with apparent HLA-A specificity reacting only against phytohemagglutinin-activated cells. La Ricerca Clin. Lab. 16, 449–456 (1986). https://doi.org/10.1007/BF02886740
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DOI: https://doi.org/10.1007/BF02886740