Summary
In this paper are reported the results obtained by a simple, reproducible and sensitive rT3 RIA, in unextracted serum, using the PEG separation technique. The linear correlation coefficient of the results obtained from extracted and unextracted sera, in a population of 60 adults and 35 child controls was 0.984, with a minimal spread of the data.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bermudez F., Surks M. I., Oppenheimer J. H.: High Incidence of Decreased Serum Triiodothyronine Concentrations in Patients with Non-Thyroid Disease — J. clin. Endocr.41, 27, 1975.
Buergi H., Wimpfheimer C., Burger A., Zaunbaure W., Roesler H., Lemarchand-Béraud Th.: Changes of Circulating Thyroxine, Triiodothyronine and Reverse Triiodothyronine after Radiographic Contrast Media — J. clin. Endocr.43, 1203, 1976.
Burger A., Nicod P., Suter P., Vallotton M. B.: Reduced Active Thyroid Hormone Levels in Acute Illness — Lanceti, 653, 1976.
Burman D. K., Read J., Dimond R. C., Strum D., Wright F. C., Patow W., Earll J. M., Warifsky L.: Measurement of 3,3′,5′-Triiodothyronine (Reverse T3), 3,3′-L-Diiodothyronine, T3, and T4 in Human Amniotic Fluid and in Cord and Maternal Serum — J. clin. Endocr.43, 1351, 1976.
Burr W. A., Griffiths R. S., Black E. G., Hoffenberg R., Meinhold H., Wenzel K. W.: Serum Triiodothyronine and Reverse Triiodothyronine Concentration after Surgical Operation — Lancetii, 1277, 1975.
Chopra I. J.: A Radioimmunoassay for Measurement of 3,3′,5′-Triiodothyronine (Reverse T3) — J. clin. Invest.54, 583, 1974.
Chopra I. J.: An Assessment of Daily Production and Significance of Thyroidal Secretion of 3,3′,5′-Triiodothyronine (Reverse T3) in Man — J. clin. Invest.58, 32, 1976.
Chopra I. J., Chopra V., Smith S. R., Renza M., Salomon D. H.: Reciprocal Changes in Serum Concentration of 3,3′,5′-Triiodothyronine (Reverse T3) and 3,3′,5-Triiodothyronine in Systemic Illness — J. clin. Endocr.41, 1043, 1975.
Chopra I. J., Crandall B. F.: Thyroid Hormones Thyrotropin in Amniotic Fluid — New Engl. J. Med.293, 740, 1975.
Chopra I. J., Sack J., Fisher D. A.: 3,3′,5′-Triiodothyronine (Reverse T3) and 3,3′,5-Triiodothyronine (T3) in Fetal and Serum Adult Sheep: Studies of Metabolic Clearance Rates, Production Rates, Serum Binding and Thyroidal Content Relative to Thyroxine — Endocrinology97, 1080, 1975.
Chopra I. J., Sack J., Fisher D. A.: Circulating 3,3′,5′-Triiodothyronine (Reverse T3) in the Human Newborn — J. clin. Invest.55, 1137, 1975.
Fisher D. A.: Reverse Triiodothyronine and Fetal Thyroid Status — New Engl. J. Med.293, 770, 1975.
Fisher D. A., Burrow G. N., Dussault J. H., Hollingsworth D. R., Larsen P. R., Man E. B., Walfish P. G.: Recommendations for Screening Programs for Congenital Hypothyroidism. Report of a Committee of the American Thyroid Association — Amer. J. Med.61, 932, 1976.
Griffiths R. S., Black E. G., Hoffenberg R.: Measurement of Serum 3,3′,5′-(Reverse) T3 with Comment on Its Derivation — Clin. Endocr.5, 679, 1976.
Harrington J. C., Fenton J. W., Pert J. H.: Polymer-Induced Precipitation of Antigen-Antibody Complexes: ‘Preciplex’ Reactions — Immunochemistry8, 413, 1971.
Kaplan M. M., Schimmel M., Utiger D. R.: Changes in Serum 3,3′,5′-Triiodothyronine (Reverse T3) Concentrations with Altered Thyroid Hormone. Secretion and Metabolism — Clin. Endocr.45, 447, 1977.
Koedding R., Janzen J., Schmidt E., Hesch R. D.: Reverse Triiodothyronine in Liver Disease (Letter) — Lancetii, 314, 1976.
MacFaul R., Grant D. B.: Early Detection of Congenital Hypothyroidism — Arch. Dis. Childh.52, 87, 1977.
Meinhold H., Wenzel K. W., Schuernbrand P.: Radioimmunoassay of 3,3′,5′-Triiodo-L-Thyronine (Reverse T3) in Human Serum and Its Application in Different Thyroid States — Z. klin. Chem.13, 571, 1975.
Nicod P., Burger A., Staheli W., Vallotton M. B.: A Radioimmunoassay for 3,3′,5′-Triiodo-L-Thyronine (Reverse T3) in Unextracted Human Serum: Methods and Clinical Results — J. clin. Endocr.42, 823, 1976.
Pittman J. A., Tingley J. O., Nickerson J. F., Hill S. R.: Anti-Metabolic Activity of 3,3′,5′-Triio-DL-Thyronine in Man — Metabolism9, 293, 1960.
Ratcliffe W. A., Marshall J., Ratcliffe J. G.: The Radioimmunoassay of 3,3′,5′-Triiodothyronine (Reverse T3) in Unextracted Human Serum — Clin. Endocr.5, 631, 1976.
Roche J., Michel R., Nunez J., Wolf W.: Sur la présence dans le plasma de la 3,3′,5′ rT3 — C. R. Soc. Biol. (Paris)150, 20, 1956.
Vagenakis A. G., Burger A., Portnay G. I., Rudolph M., O’Brian J. T., Azizi F., Arky R. A., Nicod P., Ingbar S. H., Braverman L. E.: Diversion of Peripheral Thyroxine Metabolism from Activating to Inactivating Pathways during Complete Fasting — J. clin. Endocr.41, 191, 1975.
Wartofsky L., Burman D. K., Dimond R. C., Noel G. L., Frantz A. G., Earll J. M.: Studies on the Nature of Thyroid Suppression during AcuteFalciparum malaria: Integrity of Pituitary Response to TRH and Alteration in Serum T3 and Reverse T3 — J. clin. Endocr.44, 85, 1977.
Westgren U., Burger A., Levin K., Melander A., Nilsson G., Pettersson U.: Divergent Changes of Serum 3,5,3′-Triiodothyronine and 3,3′,5′-Triiodothyronine in Patients with Acute Myocardial Infarction — Acta med. scand.201, 269, 1977.
Westgren U., Melander A., Ingemansson S., Burger A., Tibblin S., Waehlin E.: Secretion of Thyroxine, 3,5,3′-Triiodothyronine and 3,3′,5′-Triiodothyronine in Euthyroid Man — Acta endocr.84, 281, 1977.
Wilcox R. G.: Triiodothyronine, TSH and Prolactin in Obese Women — Lanceti, 1027, 1977.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bagni, B., Malavasi, F., Cavallini, A.R. et al. A 3,3′,5′-triiodothyronine (reverse T3) radioimmunoassay. La Ricerca Clin. Lab. 7, 386–395 (1977). https://doi.org/10.1007/BF02886651
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02886651