Summary
The apoptosis of lens epithelial cells (LECs) induced by ultraviolet and the expression of P53 were investigated. Wistar rats received 100 mW/m2 ultraviolet irradiation (UVR) (λ=280 nm-315 nm) for 15 min. One, 6, 24 h after irradiation the lens capsules were dissected. The percentages of apoptotic cells were evaluated by the TdT-dUTP terminal nick-end labeling (TUNED technique and the expression of P53 was detected by using immunohistochemical assay. The results showed that the percentages of TUNEL-positive nuclei at 24 h after irradiation was significantly higher than in the control group and those 1 h. 6 h after irradiation. The percentages of P53-positive cells at 6 h, 24 h after irradiation were significantly higher than in the control group and those 1 h after irradiation. It was concluded that UVR could induce the apoptosis of lens epithelial cell. The expression of P53 might be responsible for the apoptosis of lens epithelial cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Li W C, Kuszak J R, Wang G M. Calaimycin-induced lens epithelial cell apoptosis contributes to cataract formation. Exp Eye Res, 1995,16:91
Gavrieli Y, Sherman Y, Ben-Sasson S A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol, 1992,119:493
Lane D P. P53, guardian of the genome. Nature, 1992,358:15
Kastan M B, Onyekwere O. Participation of P53 protein in the cellular response to DNA damage. Cancer Res, 1991,51:6304
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Xufang, S., Weiyu, Z. & Changsong, Z. Expression of P53 during lens epithelial cell apoptosis induced by ultraviolet. Current Medical Science 21, 263–264 (2001). https://doi.org/10.1007/BF02886449
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02886449