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The effect of interleukin-6 on the proliferation of prostate cancer cellsin vitro and the modulation of this procedure

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Summary

The role of interleukin-6 (IL-6) in the growth of an androgen-independent prostate cancer cell line (PC-3m) was defined and the effect of dexamethasone, which was previously shown to modulate IL-6/IL-6 receptor (IL-6R) on this procedure was investigated. By using a pretty sensitive and specific enzyme immunoassay (ELISA), it was found that PC-3m produced certain IL-6, but there was no difference in IL-6 secretion between the group with or without dexamethasone treatment. It was also found that PC-3m cells could not be stimulated to grow by exogenous IL-6 (P>0. 05), while it could be inhibited to grow by anti-IL-6 monoclonal antibody and dexamethasone with a dose-dependent fashion. Our observation indicated that IL-6 acted as an autocrine growth factor for PC-3m, and dexamethasone could inhibit cell proliferation by a mechanism independent of its effect on IL-6 mRNA expression.

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Authors and Affiliations

  1. Department of Urology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Xing Yifei (Doctor in Charge), Xiao Yajun, Zhang Qijun & Lu Gongcheng

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  1. Xing Yifei
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  2. Xiao Yajun
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  3. Zhang Qijun
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  4. Lu Gongcheng
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Additional information

This project was supported by a grand from MSD Foundation of Ministry of Health of China.

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Yifei, X., Yajun, X., Qijun, Z. et al. The effect of interleukin-6 on the proliferation of prostate cancer cellsin vitro and the modulation of this procedure. Current Medical Science 21, 225–227 (2001). https://doi.org/10.1007/BF02886436

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  • DOI: https://doi.org/10.1007/BF02886436

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