Skip to main content
SpringerLink
Log in

Experimental study on AT1-receptor-peptide-induced myocardial immune damage in rat

  • Published:
Current Medical Science Aims and scope Submit manuscript

Summary

In order to investigate the immunological damage in rat immunized with ATI-receptor peptide. 18 male Wistar rats were divided into two groups: immunized-group (n=12), each rat was immunized with 150 μg AT1-receptor petide coupled to bovine serum albumin, together with Freund’s adjuvant. Control group (n=6), sham-immunized, “immunized liquid” was same as immunized-group except ATI-receptor peptide. Systolic blood pressure (SBP) was measured by using the tail-cuff technique, antibody against ATI-receptor peptide detected by using ELISA method, and left ventricular myocardium and renal cortex sections were observed under light and electron microscopy. There was no significant difference in SBP and light microscopic observation of the tissue sections between the immunized-group and control group. The O.D. value of anti-ATI-receptor peptide antiserum was significantly higher in the immunized-group than in the rats before immunization and control group (P<0.01). Positive rate in the immunized-group was 100%, while 0% in the control group. Ultramicroscopic morphology showed potential myocardial injury, including: increase in number of mitochondria, swelling of many mitochondria with reduction in number or absence of their cristae and cristolysis, disorder of the cardiac myofibrils, and myofibrillar disruption and myocytolysis. And lysosomes were increased in renal tubular epithelia. The ATI-receptor peptide could induce to generate the antibody against ATI-receptor peptide and lead to myocardial and renal damage in rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wallukat G, Fu H M, Matsui Set al. Autoantibodies against M2 muscarinic receptors in patients with cardiomyopathy display non-desensitized agonist-like effects. Life Sci, 1999,64:465

    Article  CAS  PubMed  Google Scholar 

  2. Liao Y H, Cheng L X, Tu YSet al. Mechanism of antibeta-adrenoceptor antibody mediated myocardial damage in dilated cardiomyopathy. J Tongji Med Univ, 1997, 17:5

    CAS  PubMed  Google Scholar 

  3. Liao Y H, Cheng L X, Dai S Pet al. Autoantibodies against ADP/ATP carrier from patients with dilated cardiomyopathy increase activity of voltage-dependent Ca2+ channels in cardiac myocytes. Blood Press Supple, 1996, 3:41

    CAS  Google Scholar 

  4. Liao Y H. Functional analysis of autoantibodies against ADP/ATP carrier from dilated cardiomyopathy. Int J Cardiol, 1996, 54:165

    Article  CAS  PubMed  Google Scholar 

  5. Dzielak D J. Immune mechanisms in experimental and essential hypertension. Am J Physiol, 1991, 29:R459

    Google Scholar 

  6. Fu M L, Herlitz H, Wallukat Get al. Functional autoimmune epitope on alpha 1-adrenergic receptors in patients with malignant hypertension. Lancet, 1994, 344: 1660

    Article  CAS  PubMed  Google Scholar 

  7. Fu M L, Herlitz H, Wallulat Get al. Non-desensitized positive chronotropic effect of anti-angiotensin I receptor autoantibodies in patients with malignant hypertension. Circulation, 1996, 94:4046a

    Google Scholar 

  8. Wallukat G, Homuth V, Fischer Tet al. Patients with preeclampsia develop agonistic autoantibodies against the angiotensin AT1 receptor. J Clin Invest, 1999, 103:945

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Liao Y H, Wei Y M, Wang M. Relationship between antoantibody against AT1-receptors and angiotesin I in patients with refractory hypertension. J Renin Angio Aldost System, 2000,1:57

    Google Scholar 

  10. Hoebeke J. Structural basis of autoimmunity against G protein coupled membrane receptors. Int J Cardiol, 1996,54:103

    Article  CAS  PubMed  Google Scholar 

  11. Matsui S, Fu M L. Myocardial injury due to G-protein coupled receptor-autoimmunity. Jpn Heart J, 1998,39: 261

    Article  CAS  PubMed  Google Scholar 

  12. 1998. 10:P9–18

  13. Fu M L, Schulze M, Mallukat Get al. Immunohistochemical localization of angiotensin I receptors (AT1) in the heart with anti-peptide antibodies showing a positive chronotropic effect. Receptors Channels, 1998, 6(2):99

    CAS  PubMed  Google Scholar 

  14. Mariac, Camilion de Hurtado, Bernardo Vet al. Angiotensin activates Na+-independent Cl-HCO3 exchange in ventricular myocardium. Circ Res, 1998,82:473

    Article  Google Scholar 

  15. Watanabe A, Endoh M. Relationship between the increase in Ca2+ transient and contrastile force induced by angiotensin I in aequorin-loaded rabbit ventricular myocardium. Cardiovasc Res, 1998,37(2):524

    Article  CAS  PubMed  Google Scholar 

  16. Kabour A, Henegar J R, Janicki J S. Angiotensin I (A I)-induced myocyte necrosis: role of the A I receptor. J Cardiovasc Pharmacol, 1994,23(4):547

    Article  CAS  PubMed  Google Scholar 

  17. Cigola E, Kajstura J, Li Bet al. Angiotensin I activates programmed myocyte cell deathin vitro. Exp Cell Res, 1997,231(2):363

    Article  CAS  PubMed  Google Scholar 

  18. Fu M L, Leung P S, Wallukat Get al. Agonist-like activity of antibodies to angiotensin I receptor subtype 1 (AT1) from rats immunized with AT1 receptor peptide. Blood Press, 1999,8(5–6):317

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cardiology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Luo Yusheng (Doctor in Charge), Liao Yuhua, Wang Min, Wei Yumiao, Dong Jihua, Wang Jinping & Lu Yingping

Authors
  1. Luo Yusheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liao Yuhua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wang Min
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei Yumiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dong Jihua
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wang Jinping
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lu Yingping
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yusheng, L., Yuhua, L., Min, W. et al. Experimental study on AT1-receptor-peptide-induced myocardial immune damage in rat. Current Medical Science 21, 198–201 (2001). https://doi.org/10.1007/BF02886428

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02886428

Key words

Search

Navigation