Summary
A novel variant of human vascular endothelial growth factor (h’VEGF165) cDNA was amplified by nested PCR method from the HL601 cells and was cloned into a eukaryotic expressing vector pcDNA3 to construct a recombinant plasmid pCD-h’VEGF165. The amplified h’VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into a eukaryotic expressing vector pcDNA3 by using the same methods. The results of DNA sequencing showed that h’VEGF165 cDNA cloned from HL601 was 600 bp in size with 8 % of the base sequence in h’VEGF165 cDNA being changed as compared with the base sequence in the wild-type hVEGF165 cDNA. The results of sequencing of hVEGF165 which was cloned from HL60 by us were consistent with the reports completely.
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References
Ferrara N, Davis-Smyth T. The biology of vascular endothelial growth factor. Endo Rev, 1997,18(1):4
Leung D W, Cachianes G. Kuang W Jet al. Vascular endothelial growth factor is a secreted angiogenic mitogen. Science, 1989,246:1306
Edmund T, Richard Mitchell, Taymer Hartmanet al. The human gene for vascular endothelial growth factor. J Biol Chem, 1991, 266:11947
Takeshita S, Tsurumi Y, Couffinahl Tet al. Gene transfer of naked DNA encoding for three isoforms of vascular endothelial growth factor stimulates collatenal development in vivo. Lab Invest, 1996,75:487
Laitinen M, Hartikainen J, Hiltune MOet al. Cathetermediated vascular endothelial growth factor gene transfer to human coronary arteries after angioplasty. Human Gene Therapy, 2000,11(2):263
Ke L D, Fueyo J, Chen Xet al. An novel approach to glioma therapy: down-regulation of the vascular endothelial growth factor in glioma cells using ribozymes. Int J Onco, 1998, 12(6):1396
Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning. A Laboratory Manual. 2nd ed. New York; Cold Spring Harber Laboratory Press, 1989, 1:21
Lei J X, Jiang A X, Pei D Q. Identification and characterization of a new splicing variant of vascular endothelial growth factor: VEGF183. Biochimica et Biophysica Acta, 1998,1443:400
Banks R E, Forbes M A, Searles Jet al. Evidence for the existence of a novel regnancy-associated soluble variant of the vascular endothelial growth factor, Receptor Flt-1. Mol Hum Report, 1998, 4(4):377
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Jianli, G., Shen, Q. Cloning and identification of a novel variant of human vascular endothelial growth factor. Current Medical Science 21, 191–194 (2001). https://doi.org/10.1007/BF02886426
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DOI: https://doi.org/10.1007/BF02886426