Summary
The ligand-binding domain of VLDL receptor contains eight imperfectly similar repeats. To discuss the contribution of each repeat to ligand binding, the RT-PCR technique was used to clone the VLDLR-cDNA from the heart muscle of Chinese people. Two recombinants were further constructed, which contained the full-length cDNA of VLDLR and the mutant lacking repeats 1–5. CHO cell line was transfected with two recombinants. The expression of VLDLR gene could be detected by RT-PCR from the CHO cells transfected with pCD-VR. The results of binding experiments showed that the ability of the CHO cells transfected with the full-length cDNA of VLDL-R binding Dil-labeled β-VLDL was higher than that of the CHO cells transfected with the mutant. Our findings indicated that human VLDL-R gene could be expressed effectively on CHO cells, and the receptor was almost inactivated when repeatsl-5 were deleted.
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References
Mehta, K D, Chen W J, Goldstein J L et al. The low density lipoprotein receptor in Xenopus laevis. I. Five domains that resemble the human receptor. J Biol Chem, 1991, 266: 10406.
Yamamoto, T, Davis C G, Brown MS et al. The human LDL receptor: A cysteine-rich protein with multiple aulsequences in its mRNA. Cell, 1984,: 39, 27
Yamamoto T, Bishop R W, Brown M S et al. Deletion in cysteine-rich region of LDL receptor impedes transport to cell surface in WHHL rabbit. Science, 1986: 232, 1230
Lee L Y, Mohler W A, Schafer B L et al. Nucleotide sequence of the rat low density lipoprotein receptor cDNA. Nucleic Acids Res, 1989: 17, 1259
Takahashi S, Kawarabayasi Y, Nakai Tet al. Rabbit very low density lipoprotein receptor: a low density lipoprotein receptor like protein with distinct ligand specificity. Proc Natl Acad Sci USA, 1992, 89: 9252
Esser V, Limbird L E, Brown M S et al. Muational analysis of the ligand binding domain of the low density lipoprotein recetpor. Biol Chem, 1988, 263:13283
Russelt D W, Brown M S, Goldstein J L. Different combinations of cysteine-rich repeats mediate binding of low density lipoprotein receptor to two different proteins. J Biol Chemm, 1989, 264:21682
Sakai J, Hoshino A, Takahashi Set al. J Biol Chem, 1994, 269, 2173
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Shen, Q., Ning, F., Zhiguo, L. et al. The binding ability analysis of the normal VLDL receptor and its mutant. Current Medical Science 21, 177–180 (2001). https://doi.org/10.1007/BF02886422
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DOI: https://doi.org/10.1007/BF02886422