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Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

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Chinese Science Bulletin

Abstract

A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g010 was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.

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Authors and Affiliations

  1. Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223, Kunming, China

    Bo Ding, Peng Shi, Jinggong Xiangyu & Yaping Zhang

  2. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100080, Beijing, China

    Dayuan Chen, Qingyuan Sun, Guangpeng Li, Minkang Wang, Yilong Liu, Zhiming Han, Xiangfen Song & Jingsong Li

  3. Fuzhou Panda Institute Center, 350001, Fuzhou, China

    Yuchun Chen

Authors
  1. Bo Ding
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  2. Peng Shi
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  3. Jinggong Xiangyu
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  4. Yaping Zhang
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  5. Dayuan Chen
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  6. Qingyuan Sun
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  7. Guangpeng Li
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  8. Minkang Wang
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  9. Yilong Liu
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  10. Li Kang
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  11. Zhiming Han
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  12. Xiangfen Song
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  13. Jingsong Li
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  14. Yuchun Chen
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Corresponding author

Correspondence to Yaping Zhang.

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Cite this article

Ding, B., Shi, P., Xiangyu, J. et al. Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda. Chin.Sci.Bull. 45, 1883–1885 (2000). https://doi.org/10.1007/BF02886299

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  • DOI: https://doi.org/10.1007/BF02886299

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