Abstract
Geminivirus is a kind of single-stranded DNA virus. Experimental results from tomato golden mosaic virus (TGMV) showed that expression pattern of coat protein gene (cp) promoter was phloem specifically expressed. In this note, the studies oncp promoter of cotton leaf curl virus (CLCuV) which is found and identified recently suggest that the promoter is not phloem specifically expressed. The expressing activity ofgus gene driven by the promoter exists not only in phloem but also in mesophyll tissues and root tip meristem. Transient expression suggests thatcp promoter transactivated by AC2 shows expressing activity in mesophyll and vascular tissue of leaf vein.
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References
Xie, Y. Q., Zhu, Z., Advances in replication and transcriptional regulation of geminiviruses, Chin. J. Virol. (in Chinese), 1999, 15: 377.
Zhan, X., Haley, A., Richardson, K. et al., Analysis of the potential promoter sequence of African cassava mosaic virus by transient expression of the β-glucuronidase gene, J. Gen. Virol., 1991, 72: 2849.
Sunter, G., Bisaro, D. M., Transactivation in a geminivirus: AL2 gene product is needed for coat protein expression, Virology, 1991, 180: 416.
Sunter, G., Bisaro, D. M., Regulation of a geminivirus coat protein promoter by AL2 protein (TrAP): evidence for activation and depression mechanisms, Virology, 1997, 232: 269.
Hong, Y., Saunder, K., Hartley, M. R. et al., Resistance to geminivirus infection by virus-induced expression of dianthin in transgenic plants, Virology, 1996, 220: 119.
Needham, P. D., Atkinson, R. G., Morris, B. A. M. et al., GUS expression patterns from a tobacco yellow dwarf virus-based episomal vector, Plant Cell Rep., 1998, 17: 631.
Xie, Y. Q., Zhu, Z., Wu, Q. et al., Isolation of a strong promoter and its functional study, Acta Botanica Sinica (in Chinese), 2000, 42: 50.
Xie, Y. Q., Zhu, Z., Liu, Y. L. et al., Partial deleted complementary sense promoter from cotton leaf curl virus has strong activity, Hightechnology Letters (in Chinese), 2000(8): 1.
Harrison, B. D., Liu, Y. L., Khalid, S. et al., Detection and relationships of cotton leaf curl virus and allied whitefly-transmitted geminiviruses occurring in Pakistan, Ann. Appl. Biol., 1997, 130: 61.
Ren, Y. G., Yu, X. L., Shan, S. et al., TransientuidA gene expression in tobacco chloroplasts by induction of therice psbA promoter, J. Agri. Biotech. (in Chinese), 1995, 3: 78.
Jefferson, R. A., Assaying chimeric genes in plants: the GUS gene fusion system, Plant Mol. Biol. Rep., 1987. 5: 387.
Liu, C. M., Zhu, Z., Zhou, Z. L. et al., Obtaining of cowpea trypsin inhibitor transgenic tobacco with insect-resistance, Chinese Science Bulletin, 1992, 18: 1694.
Legavre, T., Guiderdoni, E., Peterschmit, M. et al., Leftward transcriptional activity of the maize streak virus promoter: vascular tissue specificity in transgenic rice, in Book of Abstract: 5th International Congress of Plant Molecular Biology (ed. Dean, J. F. D.), Singapore: Kluwer Academic Publishers, 1997, 9.
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Xie, Y., Zhu, Z., Liu, Y. et al. Coat protein promoter from cotton leaf curl virus is not a tissue-specifically expressed promoter. Chin.Sci.Bull. 45, 1869–1874 (2000). https://doi.org/10.1007/BF02886296
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DOI: https://doi.org/10.1007/BF02886296