Abstract
We have cloned the replicative form of thePeriplaneta fuliginosa densonucleosis virus (PfDNV) genome and determined its complete sequence. The sequence has 5 454 nucleotides (nt), the genome consists of an internal unique sequence flanked by inverted terminal repeats (201 nt). The first 122 nt at the 5′ end and the terminal 122 nt at the 3′ end of both plus and minus strands can fold into a typical hairpin structure. The genome contains seven major open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5′ half of the plus strand, whereas the others span the 5′ half of the minus strand. Two potential promoters were found at map units (m.u.) 3 and 97. Computer analysis of sequence homologies with other parvoviruses suggests that the plus strand ofPf DNV encodes very likely the nonstructural proteins and the minus strand probably encodes the structural proteins.
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References
Hu, Y., Liang, D., Huang, Y., A small spherical virus of cockroach,Periplaneta fuliginisa, J. Wuhan Univ. (in Chinese), 1991, 1: 114.
Hu, Y., Zheng, J., Iizuka, T. et al., A densovirus newly isolated from the smoky-brown cockroachPeriplaneta fuliginosa, Arch. Virol., 1994, 138: 365.
Hu, Y., Zhang, J., Zhu, L. et al., Construction of the genome clones, subclones and deletion clones of thePf DNV DNA, Virologica Sinica (in Chinese), 1996, 11: 12.
Jourdan, M., Jousset, F. X., Gervais, M. et al., Cloning of the genome of a densovirus rescue of infectious virions from recombinant plasmid in the insect hostSpodoptera littorali, Virology, 1990, 179: 403.
Sarmbmk, J., Fritsch, E. F., Maniatis, T., Molecular Cloning, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989, 1.
Bando, H., Choi, H., Ito, Y. et al., Terminal structure of a densovirus implies a hairpin termini transfer replication which is similar to the model for AAV, Virology, 1990, 179: 57.
Bensimhon, M. J., Gabarro-Arpa, J., Reiss, C. et al., Physical characteristics in eucaryotic promoters, Nucleic Acids Res., 1983, 11: 4521.
Dumas, B., Jourdan, M., Pascaud, A. M. et al., Complete nucleotide sequence of the cloned infectious genome ofJunonia coenia densovirus reveals an organization unique among parvoviruses, Virology, 1992, 191: 202.
Birnstiel, M. L., Busslinger, M., Strub, K., Transcription termination and 3′ processing: the end is in site, Cell, 1985, 41: 349.
Mount, S. M., A catalogue of splice junction sequence, Nucleic Acids Res., 1982, 10: 459.
Chen, K. C., Shull, B. C., Moses, E. A. et al., Complete nucleotide sequence and genome organization of bovine parvovirus, J. Virol., 1986, 55: 574.
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Guo, H., Zhang, J. & Hu, Y. Complete nucleotide sequence and genomic organization ofPeriplaneta fuliginosa densonucleosis virus. Chin.Sci.Bull. 45, 1782–1786 (2000). https://doi.org/10.1007/BF02886267
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DOI: https://doi.org/10.1007/BF02886267