Abstract
An F2 population developed from theXa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene,Xa-4. Some restriction fragment length polymorphism (RFLP) markers on the high-density map constructed by Harushima et al. and the amplified DNA fragments homologous to the conserved domains of plant disease resistance (R) genes were used to construct the genetic linkage map around the geneXa-4 by scoring susceptible individuals in the population.Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction (PCR) marker M55. The R gene homologous fragment marker RS13 was found co-segregating withXa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker RS13 can be applied to molecular marker-assisted selection ofXa-4 in rice breeding programs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baker, B., Zambryski, P., Staskawicz, B. et al., Signaling in plant-microbe interactions, Science, 1997, 276: 726.
Huang, N., Angeles, E. R., Domingo, J. et al., Pyramiding of bacterial blight resistance gene in rice: marker-assisted selection using RFLP and PCR, Theor. Appl. Genet., 1997, 95: 313.
Song, W. Y., Wang, G. L., Chen, L. L. et al., A receptor kinase-like protein encoded by the rice disease resistance gene,Xa-21, Science, 1995, 270: 1804.
Yoshimura, S., Yamanouchi, U., Katayose, Y. et al., Expression ofXa-1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation, Proc. Natl. Acad. Sci. USA, 1998, 95: 1663.
Wang, Z. X., Yano, M., Yamanouch, U. et al., ThePi-b gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes, The Plant Journal, 1999, 19: 55.
Zheng, K. L., Zhuang, J. Y., Wang, H. R., Performance of resistance gene pyramids to races of rice bacterial blight in Zhejiang Province, Hereditas (in Chinese) (Beijing), 1998, 20(4): 4.
Yoshimura, S., Nelson, R., Yoshimura, A. et al., RFLP mapping of the bacterial blight resistance genesXa-3 andXa-4, Rice Genet. Newsl., 1992, 9: 136.
Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, USA, 1989, 8.3–8.8.
Harushima, Y., Yano, M., Shomura, A. et al., A high-density rice genetic linkage map with 2 275 markers using a single F2 population, Genetics, 1998, 148: 479.
Ma, B. J., Wang, W. M., Zhao, B. et al., Study on the PCR marker for the rice bacterial blight resistance geneXa-4, Hereditas (Beijing), 1999, 21(3): 9.
Lander, E. S., Green, P., Abrhamson, J. et al., MAPMAKER: an interactive computer package for constmcting primary genetic linkage maps of experimental and natural populations, Genomics, 1987, 1: 174.
Kurata, N., Umehara, Y., Tanoue, H. et al., Physical mapping of the rice genome with YAC clones, Plant Molecular Biology, 1997, 35: 101.
He, P., Li, S. G., Qian, Q. et al., Genetic analysis of rice grain quality, Theor. Appl. Genet., 1999, 98: 502.
Leister, D., Ballvora, A., Salamini, F. et al., A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants, Nature Genetics, 1996, 14: 421.
Leister, D., Kurth, J., Laurie, D. A. et al., RFLP- and physical mapping of resistance gene homologues in rice (O. sativa) and Barley (H. Vulgare), Theor. Appl. Genet., 1999, 98: 509.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Wang, W., Zhou, Y., Jiang, G. et al. Fine mapping of the rice bacterial blight resistance gene Xa-4 and its co-segregation marker. Chin.Sci.Bull. 45, 1779–1782 (2000). https://doi.org/10.1007/BF02886266
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02886266