Abstract
The dynamic changes of IAA in graft union of explanted internode autografting of Cucumber (Cucumis sativus Linn.) have been investigated using the immunohistochemical localization technique. It is shown that the efficiency of IAA fixation using lyophilization-gas fixation is higher than that using liquid chemical fixation. In contrast to few silver particles and no significant changes during the development of graft union cultured in hormone-free medium, more silver particles in graft union and significant changes of IAA related to graft union development have been found when graft union was cultured in medium supplemented with appropriate hormones. The fixation procedure of plant hormones and the roles of IAA in graft union are discussed.
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Parkinson, M., Yeoman, M. M., Graft formation in cultured, explanted internodes, New Phytol., 1982, 107: 489.
Lu, S. F., Tang, D. T., Song, J. Y. et al., Preliminary studies on controlling graft union through plant hormones, Acta Bot. Sin. (in Chinese), 1996, 38: 307.
Lu, S. F., Song, Y. R., Hormonal regulation of vascular tissue differentiation in graft union, Acta Bot. Yunnan (in Chinese), 1999, 21: 483.
Lu, S. F., Song, Y. R., Relation between phytohormone level and vascular bridge differentiation in graft union of explanted internode autografting, Chinese Science Bulletin, 1999, 44(20): 1874.
Zhang, J., Han, B. W., Advance in the study of histochemical localization for plant hormones, Chin. Bull. Bot. (in Chinese), 1995, 12(suppl.): 131.
Hasegawa, M., Hashimoto, N., Zhang, J. et al., Immunohistochemistry of gibberellins in rice anthers, Biosci. Biotech. Biochem., 1995, 59: 1925.
Jia, W. S., Huang, C. L., Zhang, D. P., Immuno-gold localization of IAA in the leaf cells of Vicia faba, Acta Bot. Sin. (in Chinese), 1997, 39: 596.
Ohmiya, A., Hayashi, T., Kakiuchi, N., Immuno-gold localization of indole-3-acetic acid in peach seedings, Plant Cell Physiol., 1990, 31: 711.
Ohmiya, A., Hayashi, T., Immuno-gold localization of IAA in leaf cells of Prunus persica at different stages of development, Physiol. Plant., 1992, 85: 439.
Shi, L., Miller, I., Moore, R., Immunocytochemical localization of indole-3-acetic acid in primary roots of Zea mays, Plant Cell Environ., 1993, 16: 967.
Jones, A. M., Location of transported auxin in etiolated maize shoots using 5-azidoindole-3-acetic acid, Plant Physiol., 1990, 93: 1154.
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Lu, S. Immunohistochernical localization of IAA in graft union of explanted internode grafting. Chin.Sci.Bull. 45, 1767–1771 (2000). https://doi.org/10.1007/BF02886263
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DOI: https://doi.org/10.1007/BF02886263