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Isolation and cloning of a novel cDNALDB1 encoding human LIM domain binding protein

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Chinese Science Bulletin

Abstract

Primers for screening cDNA library have been designed according to EST AA453734 which is corresponding to the mouse LIM domain binding protein Ldbl. Arrayed human fetal brain cDNA library has been screened by PCR and routine hybridization method. A 2398 bp-cD-NA clone has been obtained. The cDNA encodes a 347 amino acids protein highly homologous to the mouse Ldbl,Xenopus Xldbl andDrosophila Chip. It also contains an LIM binding domain and a nuclear localization signal. It has been namedLDB1 ( LIM domain binding protein 1), GenBank accession number is AF052389. Northern blot showed a 2.4 kb band, and the expression amounts ofLDBI in heart, brain and lung were considerably higher than those in other tissues.

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Authors and Affiliations

  1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 100005, Beijing, China

    Yan Zhou, Guangwei Du, Junhua Wang, Jianhe Chen, Jiangang Yuan & Boqin Qiang

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  1. Yan Zhou
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  2. Guangwei Du
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  3. Junhua Wang
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  4. Jianhe Chen
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  5. Jiangang Yuan
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  6. Boqin Qiang
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Zhou, Y., Du, G., Wang, J. et al. Isolation and cloning of a novel cDNALDB1 encoding human LIM domain binding protein. Chin.Sci.Bull. 44, 1114–1119 (1999). https://doi.org/10.1007/BF02886138

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  • DOI: https://doi.org/10.1007/BF02886138

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