Abstract
The induction of apoptosis in suspension culture of tobacco cells by heat shock is reported for the first time. Heat treatment (48° for 4 h) of tobacco cells led to the appearance of typical hallmarks of apoptosis. It was demonstrated by DNA laddering analysis that the cells treated with heat shock at 48°C for 4 h had a serious degradation of nuclear DNA into multi-nucleosomal sizes, suggesting that heat shock activated endogenous nuclease which led to DNA cleavage at the linkage sites between the nucleosomes, but ladders were very faint for DNA from2 and 9 h heat-treated cells. The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) detection also showed that most of these treated cells (48°C for4 h) displayed positive reactions, indicating a serious DNA 3′-OH cleavage in their nuclei. Moreover, some other cytological changes in apoptotic cells, such as cell shrinkage, chromatin aggregation, nucleus collapse, have also been observed by 4′, 6′-diamidino-2-phenyl-indole (DAPI) staining.
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References
Kerr, J. F. R., Wylllie, A. H., Currie, A. H., Apoptosis: a basic biological phenomenon with wide ranging implications in tissue kinetics,Bri. J. Cancer., 1972, 26: 239.
McCabe, P. F., Levine, A., Meijer, P. J.et al., A programmed cell death pathway activated in carrot cells cultured at low density,Plant J., 1997, 12(2): 267.
Jiang, X. F., Zhu, H. Z., Zhou, J.et al., Application of comet assay in plant protoplast apoptosis detection,Acta Botanica Sinica (in Chinese), 1998, 40(10): 928.
Poccia, F., Peselli, P., Vendetti, S.et al., Heat shock protein expression on the membrane of T-cell undergoing apoptosis,Immnunology, 1996, 88: 6.
Li, W. X., Chen, C. H., Ling, C. C.et al., Apoptosis In heat induced cell killing the protective role of hsp-70 and the sensitization effect of the c-myc gene,Radh Res., 1996, 324.
Zhu, W. G., Aramaki, R., Cai, Y.et al., Promotion of heat-induced apoptosis in Fm3A cells by protease inhibitors,Biochem. Bioph. Res. Co., 1996, 225(3): 924.
Vogel, P., Dux, E., Wiessner, C., Evidence of apoptosis in primary neuronal cultures after heat-shock.,Brain Res., 1997, 764(1-2): 205.
Wang, H., Juan, L., Richard,. M. B.et al., Apoptosis: a functional paradigm for programmed plant cell death induced by a host-selective phytotoxin and invoked during development,Plant Cell. 1996, 8: 375.
Mitter, R., Lam, E., Identification, characterization, and purification of a tobacco endonuclease activity induced on hypersensitive response cell death,Plant Cell, 1995, 7: 1951.
Harm, H. K., Thermotoleranee in mammalian cells protein denaturation and aggregation, and stress proteins,J. Cell Sci., 1993, 104: 11.
Mosser, D. D., Caron, A. W., Bourget, L.et al., Role of the human hat-shock-protein Hsp70 in protection against stressinduced apoptosis,Mol. Cell Biol., 1997, 17(9): 5317.
Mehlen, P., Mehlen, A., Godet, J.et al., Hap27 as a switch between differentiation and apoptosis in murine embryonic stem-cells,J. Biol. Chem., 1997, 272(50): 31657.
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Chen, H., Yan, C., Jiang, X. et al. Apoptosis in suspension culture of tobacco cells induced by heat shock. Chin.Sci.Bull. 44, 1101–1106 (1999). https://doi.org/10.1007/BF02886135
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DOI: https://doi.org/10.1007/BF02886135