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Analysis of α1-adrenoceptor subtypes in neonatal rat heart

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Chinese Science Bulletin

Abstract

The expression levels of 3 subtypes (αlA, αlB and α1D) of α1-adrenoceptor have been determined by radioligand binding assay and reverse-transcription polymerase chain reaction (RT-PCR) assay in neonatal rat heart. mRNAs for all the 3 subtypes have been detected in RT-PCR assays. The density of total αI-AR (B max) was (134±4) fmol/mg protein and theK d value was (164 ±15) pmol/L. The α1-adrenoceptor subtypes selective antagonists, RS17053, ( + )niguldipine, 5-MU, BMY 7378 and WB4101, competitively inhibited binding of125I-BE 2254 to α1-adrenoceptor in a concentration-dependent manner. The inhibition curves were better fit for the two-site model. The binding sites for α1A-, α1B-and α1D-adrenoceptor amounted to 25%, 50% and 25% of the total binding sites, respectively.

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Authors and Affiliations

  1. Institute of Vascular Medicine, The Third Hospital, Beijing Medical University, 100083, Beijing, China

    Hong Wei, Zhizhen Lü, Youyi Zhang & Qide Han

Authors
  1. Hong Wei
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  2. Zhizhen Lü
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  3. Youyi Zhang
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  4. Qide Han
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Wei, H., Lü, Z., Zhang, Y. et al. Analysis of α1-adrenoceptor subtypes in neonatal rat heart. Chin.Sci.Bull. 44, 1098–1101 (1999). https://doi.org/10.1007/BF02886134

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  • DOI: https://doi.org/10.1007/BF02886134

Keywords: neonatal rat

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