Abstract
Resistance-like sequences have been amplified from first strand cDNA and genomic DNA of rice by PCR using oligonucleotide primers designed from sequence motifs conserved between resistance genes of tobacco andArabidopsis thaliana. 3 PCR clones, designatedOsr1, Osr2 andOsr3 which were 98% identical in nucleotide sequence level, have been found to be significantly homologous to known plant resistance genes and all contained the conserved motifs of NBS-LRR type resistance genes, such as P-loop, kinase2a, kinase3a and transmembrane domain.Southern hybridization revealed that rice resistance gene hornologueswere organized as a cluster in the genome. RFLP mapping using a DH population derived from anindica/japonka cross (Zhaiyeqing 8/Jingxi 17) and an RFLP linkage map assigned two copies ofOsrl and one copy ofOsr3 to the distal position of chromosome 12 where a blast resistance QTL has been mapped previously. Northern blot analysis showed thatOsrl gene was constitutively transcribed in rice leaves, shoots and roots. Further study concerning isolation of full-length cDNAs would be conducive to elucidating the functions of these genes.
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References
Baker, B., Zambryski, P., Staskawize, B.et al., Signaling in plant-microbe interactions,Science, 1997, 276: 726.
Zhu, L. H., Chen, Y., Ling, Z. Z.et al., Identification of molecular markers linked to a blast resistance gene in rice,Science in China, Ser. B, 1994, 24(10): 1048.
Kanazin, V., Marek, L. F., Shoemaker, R., Resistance gene analogues are conserved and clustered in soybean,Proc. Natl. Acad. Sci. USA, 1996, 93: 11746.
Yu, Y. G., Buss, G. R., Saghai-Maroof, M. A., Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site,Proc. Natl. Acad. Sci. USA, 1996, 93: 11751.
Lister, D., Ballvora, A., Salamini, F.et al., A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants,Nature Genet., 1996, 13: 421.
Lister, D., Kurth, J., Laurie, D. A.et al., Rapid reorganization of resistance gene homologues in cereal genomes,Proc. Natl. Acad. Sci. USA, 1998, 95: 370.
Xue, Y. B., Tang, D. Z., Zhang, Y. S.et al., Isolation of candidate R disease resistance genes from rice,Chinese Science Bulletin, 1998, 43(6): 497.
Wang, S. P., Liu, K. D., Wang, J.et al., Identifying candidate disease resistance genes in rice by sequence homology and chromosomal locations,Acta Botanica Sinica (in Chinese with English summary), 1998, 40: 42.
Fenillet, C., Schachermayr, G., Keller, B., Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease-resistance locus of wheat,The Plant J., 1997, 11: 45.
Ohmori, T., Murata, M., Motoyoshi, F., Characterization of disease resistance-like sequences in near-isogenic lines of tomato,Theor. Appl. Genet., 1998, 96: 331.
Yoshimura, S., Yamanouchi, U., Katayose, Y.et al., Expression of Xal, a bacterial blight resistance gene in rice, is induced by bacterial inoculation,Proc. Natl. Acad. Sci. USA, 1998, 95: 1663.
Zhang, J. S., Zhou, J. M., Zhang, C.et al., Differential gene expression in salt-tolerant rice mutant and its parental variety,Science in China, Ser. C, 1996, 39: 310.
Chen, S. Y., Zhu, L. H., Hong, J., Molecular biological identification of a rice salt-tolerant line,Acta Botanica Sinica (in Chinese with English summary), 1991, 33:569.
Bent, A. F., Kunkel, B. N., Dahlbeck, D.et al., RPS2 ofArabidopsic thaliana: a leucine-rich repeat class of plant disease resistance genes,Science, 1994, 265: 1865.
Mindrinos, M., Katagiri, F., Yu, G. L.et al., TheA. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeat,Cell, 1994: 78: 1089.
Whitham, S., Dinesh-kumar, S. P., Choi, D.et al., The product of tobacco moisaic virus resistance gene N: similarity to Toll and the interleukin-1 receptor,Cell, 1994, 78: 1101.
Wang, G. L., Mackill, D. J., Bonman, M.et al., RFLP mapping of genes conferring complete and partial reeistance to blast in a durably resistance rice cultivar,Genetics, 1994, 136: 1421.
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Li, Z., Chen, S. Molecular cloning, chromosomal mapping and expression analysis of disease resistance gene homologues in rice (Oryza sativa L.). Chin.Sci.Bull. 44, 1202–1207 (1999). https://doi.org/10.1007/BF02885966
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DOI: https://doi.org/10.1007/BF02885966