Abstract
Both enhanced were H+ transport activities of tonoplast vesicles isolated from roots of the salt-tolerant mutant and wild type of wheat with treatment of NaCl, but the activity of the mutant was significantly higher than that of wild type. H+ transport activity was indicated as the stable value of fluorescence quenching per mg membrane proteins. The H+ transport activities dependent on ATP of the mutant and wild type were 1099 and 558 respectively and their activities dependent on PPi were 358 and 228 separately.
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Jeschke, W. D., K+-Na+ exchange at cellular membranes, intracellular compartmentation of cations, and salt tolerance, inSalinity Tolerance in Plants Stratcgiu for Crop Improvment (eds. Staples, R. C., Toenniessen, G. H.), New York: Wiley-Interscience, 1984, 37.
Barkla, B. J., Blumwald, E., Identification of a 170 Kda protein associated with the vacuolar Na+/H+ ofBetavulgaris, Proc. Natl. Acad. Sci. USA, 1991, 88: 11 117.
Reuveni, M., Benett, A. B., Bressan, R. A.et al., Enhanced H+ transport capacity and AIP hydrolysis activity of the tono-plsst H+-ATPase after NaCl adaptation,Plant Physiol., 1990, 94: 524.
Ballasteros, E., Donain, J. P., Belver, A., Effects of salt stress on H+-ATPase and H+-PPase activities of tonoplast-enrichsd vesicles isolated from sunflower roots as affected by salt stress,Physiol. Plant., 1996, 97: 259.
Guo, F. Q., Li, Q., Gu, R. Q., Mutation, selection and comparison of several saline-tolerant wheat strains,Acta Agriculturae Nucleatae Sinica (in Chinese), 1997, 11(1): 1.
Wang, Y. Z., Sae, H., Similarities and differences between the tonoplast-type and the mitochondrial H+-ATPase of oat roots,J. Bio. Chem., 1985, 260: 10434.
Balleateros, E., Blumwald, E., Donire, J. P.et al., Na+/H+ antiport activity in tonoplast vesicles isolated from sunflower roots induced by NaCl stress,Physiol. Plant, 1997, 99: 328.
DuPont, F. M., Salt-induced changes in ion transport: Regulation of primary pumps and secondary transporters, inTransport and Receptor Proteins of Plant Membranes (eds. Cooke, D.T., Clarkson, D. T.), New York: Plenum Press, 1992, 91.
Haro, R., Banuelos, M. A., Quintero, F.J. et al., Genetic basis of sodium exclusion and sodium tolerance in yeast, A model for plants,Physiol. Plaru, 1993, 89: 868.
Guo, F. Q., Tang. Z. C., Difference of Na+, K+ accumulation in the salt-tolerant mutant of wheat and the wild type during exposures to NaCl stress,Acta Botanica Sinica (in Chinese), 1999.
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Guo, F., Tang, Z. Enhanced H+ transport activity of tonoplast vesicles isolated from roots of salt-tolerant mutant of wheat under NaCl stress. Chin.Sci.Bull. 44, 1198–1201 (1999). https://doi.org/10.1007/BF02885965
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DOI: https://doi.org/10.1007/BF02885965