Abstract
Traditional Chinese medicine (TCM) is a great treasure of China, the analysis of which is an arduous task. The viewpoint that all chemical constituents of Chinese herbal complex prescription should be analyzed as a black box is elucidated for the first time. Intelligent multi-mode multi-column chromatographic system (IMMCC) with its hybrids is the basic method and HPLC Unified Method is the breakthrough for the black box analysis. Dang-Gui-Bu-Xue-Tang was selected as a typical TCM and a systematic separation method from non-aqueous mobile phase to pure water mobile phase was put forward in order to convert unknown sample to known sample. The a,c values and UV spectra of 66 components ofAstragalus, 78 components ofAngelica and 71 components of Dang-Gui-Bu-Xue-Tang were obtained. Intelligent optimization and peak identification method and software for complex samples were developed and the optimum multi-step multi-binary gradient curve of mobile phase forAstragalus was ascertained. The maximum error and minimum error of predicted retention time for all components ofAstragalus are 8.62% and 0.05% respectively. All components ofAstragalus were compared with those ofAngelica and it is found that many components ofAstragalus are the same as those ofAngelica, while the contents of these components are different. Many components of Dang-Gui-Bu-XueTang are also the same as those ofAstragalus andAngelica with different contents.
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References
Chen, Y. Z., Discussion of researches and development strategy on Chinese herbal complex prescription,Research and Development on Worldwide Science and Technology (in Chinese), 1995(6): 17.
Institute of Materia Medica, Chinese Academy of Medical Science,Modern Researches on Chinese Herbal Medicine (in Chinese), Vol. 1, Beijing: Union Press of Beijing Medical University and Peking Union Medical College, 1995.
Yan, Y. Q., Enhancement of researches on Chemical constituents of Chinese herbal complex prescription,ZhongYm Tong Bao (in Chinese), 1985, 10(2): 3.
Liang, X. M., Intelligent multi-mode and multi-column chromatographic system with its hybrids,Chinese Journal of Chromatography (in Chinese), 1995, 13(5): 307.
Lu, P. C., Zhang, Y. C., Liang, X. M.,High Performance Liquid Chromatography and Its Expert System (in Chinese), Shenyang: Liaoning Science and Technology Publishing House, 1992.
Xu, G. W., Lu, P. C.,Gas Chromcrtography Expert System: Selectrion of Optimum Column System and Its Software (in Chinese), Jinan: Shandong Science and Technology Publishing House, 1994.
Ling, B. C.,Introduction to Capillery Chromatography (in Chinese), Beijing: Science Press, 1996.
Lu, P. C., Zhang X. M., Yang, L.et al., Optimization of gas chmmatographic analytical methods for toxic compounds in air,Anal. Sci., 1994, 10(4): 241.
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Xiao, H., Liang, X., Lu, P. et al. New method for analysis of Chinese herbal complex prescription and its application. Chin.Sci.Bull. 44, 1164–1172 (1999). https://doi.org/10.1007/BF02885958
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DOI: https://doi.org/10.1007/BF02885958