Abstract
Tween-80 supplementation in submerged culture ofAspergillus fumigatus resulted in an increase of phosphate uptake. The uptake system was characterized as saturable, energy-dependent and operating against the concentration gradient. Control and Tween 80 cultures showed similarK m values for phosphate uptake (50 μm). Cold osmotic shock treatment of the cultures was found to cause considerable reduction in the ability to take up phosphorus with concomitant release of the binding protein into the shock fluid. Binding protein preparation from Tween-80 supplemented cells showed more activity than that from control cells.
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Barash H., Halpern Y. S.: Glutamate binding protein and its relation to glutamate transport inEscherichia coli.Biochem. Biophys. Res. Commun. 45, 681 (1971).
Brown C. E., Romano A. H.: Evidence against necessary phosphorylation during hexose transport inAspergillus nidulans.J. Bacterial. 100, 1198 (1969).
Desai J. D., Misra Jyotsna, Modi V. V.: Phosphorus uptake in Biotin deficientAspergillus nidulans. An evidence for involvement of binding protein in this process.Indian J. Exp. Biol. 16, 378 (1976).
Dvorak H. F., Wetzel B. K., Heppel L. A.: Biochemical and cytochemical evidence for the polar concentration of periplasmic enzymes in a “Minicell” strain ofEscherichia coli.J. Bacteriol. 104, 543 (1970).
Fiske C. H., Subbarow Y. J.: The colorimetric determination of phosphorus.J. Biol. Chem. 66, 376 (1925).
Kaback H. R.: Transport.Annu. Rev. Biochem. 39, 561 (1970).
Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J.: Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265 (1951).
Mizrahi A., Miller G.: Role of glycols and Tweens in the production of ergot alkaloids byClaviceps purpurea.J. Bacteriol. 97, 1155 (1969).
Moore B. G., Spencer B.: Non-specific uptake and specific binding of sulfate byAspergillus nidulans.Biochem. J. 127, 72 72 (1972).
Pardee A. B.: Purification and properties of sulfate binding protein fromSalmonella typhimurium.J. Biol. Chem. 241, 5886 (1966).
Rao K. K., Patel V. P., Patel Bharti: Alkaloid production byAspergillu fumigatus as influenced by changes in substrate composition.Indian J. Exp. Biol. 12, 76 (1974a).
Rao K. K., Gupta A. R.: Effect of zinc and potassium ions on growth and tryptophan synthetase activity in alkaloid producing culture ofAspergillus fumigatus.Curr. Sci. 43, 415 (1974b).
Rao K. K., Gupta A. R.: Inhibition of tryptophan synthetase by high phosphate in alkaloid producing cultureof Aspergillus fumigatus.Naturwiss.62, 394 (1975a).
Rao K. K., Rao Satya: Effect of Tweens on the production of ergot alkaloids byAspergillus fumigatus.Folia Microbiol. 20, 418 (1975b).
Řeháček Z., Bassapa S. C.: Effect of Tween-80 on an alkaloid producing culture ofClaviceps purpurea.Folia Microbiol. 16, 110 (1971).
Wetzel B. K., Spicer S7. S., Dvorak H. F., Heppel L. A.: Cytochemical localisation of certain phosphatases inEscherichia coli.J. Bacteriol. 104, 529 (1970).
Wiley W. R.: Tryptophan transport inNeurospora crassa. A tryptophan binding protein released by cold osmotic shock.J. Bacteriol. 103, 656 (1970).
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Rao, K.K., Mehta, A.M. & Gupta, A.R. Phosphate uptake and involvement of binding protein in Tween-80 supplemented culture ofAspergillus fumigatus . Folia Microbiol 22, 216–221 (1977). https://doi.org/10.1007/BF02885603
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DOI: https://doi.org/10.1007/BF02885603