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Direct gene transferState of the Art and Future Potential

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Abstract

Incubation of freshly isolated protoplasts with a gene under the control of plant expression signals leads to high levels of stable integrative transformation. The gene is transmitted to sexual offspring and inherited in a Mendelian pattern. The foreign gene is in most cases stably maintained through several sexual cycles. However, cases of somatic and meiotic instability are also found. A partially optimized protocol routinely yields transformation frequencies in the percentage range. Host range limitations were not expected and also not found in transformation experiments with protoplasts from a series of herbaceous dicots and a graminaceous monocot. Co-transformation experiments with a selectable and a non-selectable gene on separate plasmids yielded high levels of cotransfer of the non-selectable gene.

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Authors and Affiliations

  1. Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland

    I. Potrykus, R. D. Shillito, M. W. Saul & J. Paszkowski

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  1. I. Potrykus
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  2. R. D. Shillito
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  3. M. W. Saul
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  4. J. Paszkowski
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Potrykus, I., Shillito, R.D., Saul, M.W. et al. Direct gene transferState of the Art and Future Potential . Plant Mol Biol Rep 3, 117–128 (1985). https://doi.org/10.1007/BF02885591

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