Skip to main content
SpringerLink
Log in

Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers

  • Notes
  • Published:
Chinese Science Bulletin

Abstract

The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation. Two populations were analyzed using cDNA-RAPD. The results showed that: (i) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (II) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. (iii) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zhang, Q. F., Shen, B. Z., Dai, X. K.et al., Using bulked extremes and recessive class to map genes for photoperiodsensitive genic male sterility in rice,Pro. Natl. Acad. Sci. USA, 1994, 91: 8675.

    Article  CAS  Google Scholar 

  2. Wang, B., Xu, W. W., Wang, J. Z.et al., Tagging and mapping the thermo-sensitive genic male-sterile gene in rice (O-ryza sativa L.) with molecular markers,Theor. Appl. Genet., 1995, 91: 1111.

    CAS  Google Scholar 

  3. Straus, D., Ausubel, F. M., Genomic subtraction for cloning DNA corresponding to deletion mutations,Proc. Natl. Acad. Sci, USA, 1990, 87: 1889.

    Article  PubMed  CAS  Google Scholar 

  4. Liang, P., Pardee, A. B., Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction,Science, 1992, 257(14): 967.

    Article  PubMed  CAS  Google Scholar 

  5. Hubank, M., Schatz, D. G., Identifying difference analysis of cDNA,Nucleic Acids Research, 1994, 22(25): 5640.

    Article  PubMed  CAS  Google Scholar 

  6. Yoshida, K. T., Naito, S., Takeda, G., cDNA cloning of regeneration-specific genes in rice by differential screening of randomly amplified cDNAs using RAPD primers,Plant Cell Physiol., 1994, 35(7): 1003.

    PubMed  CAS  Google Scholar 

  7. Michelmore, R. W., Paran, I., Kesseli, V., Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations,Proc. Natl. Acad. Sci. USA, 1991(88): 9828.

Download references

Author information

Authors and Affiliations

  1. Biotechnology Research Center, Chinese Academy of Agricultural Science, 100081, Beijing, China

    Shuye Jiang & Xuanjun Fang

  2. Institute of Genetics and Crop Breeding, Fujian Agricultural University, 350002, Fuzhou, China

    Shuye Jiang & Qifeng Chen

Authors
  1. Shuye Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qifeng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xuanjun Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Jiang, S., Chen, Q. & Fang, X. Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers. Chin.Sci.Bull. 44, 348–351 (1999). https://doi.org/10.1007/BF02885489

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02885489

Keywords

Search

Navigation