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Changes in the activity of glucose-6-phosphate dehydrogenase and some problems relating to its regulation in tobacco plants infected with potato virus Y

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Biologia Plantarum

Abstract

The contents of NADP+, NADPH, changes in the activity of glucose-6-phosphate dehydrogenase and some questions relating to its regulation in leaf tissues of tobacco plants infected with PVY were studied. The content of NADP+ and the total sum of pyridine nucleotides decreased after inoculation to 15 % and 30 %, respectively, whereas the content of NADPH increased up to the threefold control value. The contents of NADP+, NADPH and Σ(NADPH + NADP+) linearly correlated with the reproduction curve of PVY. The value of the reduction charge RC and the value of the ratio NADPH/NADP+ sharply increased after inoculation up to tenfold and eighteenfold values, respectively, of the healthy control.

The activity of glucose-6-phosphate dehydrogenase was markedly increased in virus-infected tissues during the entire experimental period both in crude homogenate and after its partial purification when compared with the values found in healthy control plants. The time courses of the activity curves of both crude and partially purified enzymes were coincident and correlated with the reproduction curve of PVY. The results indicate the involvement of coarse regulation of the activity of the enzyme by its content without the involvement of fine regulation by the ratio NADPH/NADP+ and RC.

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  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na Karlovce 1, 160 00, Praha 6, Czechoslovakia

    L. Šindelár

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  1. L. Šindelár
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Šindelár, L. Changes in the activity of glucose-6-phosphate dehydrogenase and some problems relating to its regulation in tobacco plants infected with potato virus Y. Biol Plant 28, 440–448 (1986). https://doi.org/10.1007/BF02885048

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  • DOI: https://doi.org/10.1007/BF02885048

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