Abstract
This paper describes the differentiation of karyotypically stable callus strains ofCrepis capillaris (with modal chromosome numbers 7, 8 –11 and ≧ 12, in particular) during 12 years’ subculturing on an identical M-S medium with supplements, including 2,4-D (1 mg 1−1) and adenine (1 mg 1−1). The possible modes and factors of karyotypic adaptationin vitro are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bayliss, M. W.: Chromosome number variation in cultured plant cells. - Nature246: 529–530, 1973.
Bayliss, M. W.: The effects of growthin vitro on the chromosome complement ofDaucus carota (L.) suspension cultures. -Chromosoms51: 401–411, 1975.
Demoise, F., Partanen, C. R.: Effects of subculturing and physical condition of medium on the nuclear behavior of a plant tissue culture. - Amer. J. Bot.56: 147–152, 1969.
Döbel, P.: Verringerung des Ploidiegrades und der Restitutionskernbildung in einer gegen hohe 2,4-D-Konzentrationen resisten Suspensionskultur vonNigella damascena. - Biol. Zentralbl.102: 431–444. 1983.
Ghosh, A., Gadgil, V. N.: Shift in ploidy level of callus tissue: a function of growth substances. - Indian J. exp. Biol.17: 562–564, 1979.
Jha, T. B., Rot, S. C.: Chromosomal behaviour in cultures ofVicia faba. - Cytologia47: 465 to 470, 1982.
Kallak, H.: [On karyotypic differentiation of callus cells in long-term culture.] In Russ. - Acta Commentat. Univ. tartu.583: 25–36, 1983.
Kallak, H. I., Järvekülg, L. J.: [On nuclear form and division in plant tissue culture.] In Russ. -Acta Commentat. Univ. tart.383: 52–63, 1976.
Kallak, H., Järvekülg, L.: Changes in chromosome complement in long-term pea callus cultures. -Acta biol. Acad. Sci. hung.28: 183–189, 1977.
Kallak, H., Vapper, M.: [Cytogenetic characteristic of long-term callus culture ofCrepis capillaris.] In Russ. - Acta Commentat. Univ. tartu.499: 18–29, 1979.
Kallak, H. I., Vapper, M. A.: Plant tissue culture as a model system for mutagenicity testing of chemicals. -Mutation Res.147: 51–57, 1985.
Kordyum, E. L., Nedukha, E. M., Sidorenko, P. G.: [Structural and functional characteristics of plant cell in processes of differentiation and dedifferentiation.] In Russ. - Naukova Dumka, Kiev 1980.
Kunakh, V. A.: [Cytogenetic differences in strains of tissue cultures of leaf and stem origin inHaplopappus gracilis (Nutt.) Gray.] In Russ. - Cytol. Genet.5: 241–249, 1971.
Melchers, G.: Einige genetische Gesichtspunkte zu sogenannten Gewebekulturen. - Ber. deut. bot. Ges.78: 21–29, 1965.
Mitra, J., Steward, F. C.: Growth induction in cultures ofHaplopappus gradlis. II The behavior of the nucleus. - Ámer. J. Bot.48: 358–368, 1961.
Novák, F. J.: The changes of karyotype in callus cultures ofAllium sativum L. - Caryologia27: 45–54, 1974.
Novák, F. J.: Chromosomal characteristics of long-term callus cultures ofAllium sativum L. - Cytologia46: 371–379, 1981.
Partanen, C. R.: Quantitative chromosome changes and differentiation in plants. - In: Rudnick, D. (ed.): Developmental Cytology. Pp. 21–45. New York 1959.
Roy, S. C.: Chromosomal variations in the callus tissues ofAllium tuberosum andA. cepa. - Protoplasma102: 171–176, 1980.
Sacristan, M. D.: Karyotypic changes in callus cultures from haploid and diploidplants ofCrepis capillaris (L.) Wallr. -Chromosoma33: 273–283, 1971.
Sacristan, M. D.: Clonal development in tumorous cultures ofCrepis capillaris. - Naturwissenschaften62: 139–140, 1975.
Shamina, Z. B.: Cytogenetic study of tissue culture ofHaplopappus gracilis. - In: Mechanism of Mutation and Inducing Factors. (Proc. of the Symp. on the Mutational Process 9–11 August 1965, Prague). Pp. 377–380. Czechosl. Acad. Sci., Praha 1966.
Shamina, Z. B., Frolova, L. F.: [Cytogenetic studies on tissue culture ofHaplopappus during prolonged cultivation.] In Russ. - In: Butenko, R. G. (ed.): The Culture of Isolated Plant Organs, Tissues and Cells. (Proc. of the 1st National Conference 22 – 26 January1968, Moscow). Pp. 149–154. Moscow 1970.
Singh, B.D.: Evolution of dominant karyotypes inHaplopappus gradlis cells culturedin vitro, - Caryologia28: 29–37, 1975.
Singh, B. D.: Variation in chromosome number and structure in plant cells duringin vitro culture. - In: Plant Tissue and Cell Culture -Application to Crop Improvement. (Abstracts of International Symposium 24 – 29 Sept. 1984, Olomouc, Czechoslovakia). Pp. 175. Olomouc 1984.
Singh, B. D., Harvey, B., Kao, K., Miller, R.: Selection pressure in cell populations ofVicia hajastana culturedin vitro. - Can. J. Genet. Cytol.14: 65–70, 1972.
Singh, B. D., Harvey, B. L.: Cytogenetic studies onHaplopappus gracilis cells cultured on agar and in liquid media. - Cytologia40: 347–354, 1975.
Sunderland, M.: Nuclear cytology. - Bot. Monogr.11: 161–190, 1973.
Torrey, J. G.: Cytological evidence of cell selection by plant tissue culture media. - In:White, P. R., Grove, A. R. (ed): (Proceedings of Internat. Conference on Plant Tissue Culture). Pp. 449–462. Mc Cutchan Publishing Co., Berkeley 1965.
Zosimovich, V. P., Levenko, B. A., Yurkova, G. N., Legeïda, V. S.: [Isolation ofCrepis capillaris strains with different ploidy level in tissue culture.] In Russ. - Dokl. Akad. Nauk SSSR203: 1188–1189, 1972.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vapper, M., Kallak, H. Karyotypic differentiation of long-term callus culture ofCrepis capillaris . Biol Plant 28, 417–423 (1986). https://doi.org/10.1007/BF02885044
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02885044