Abstract
The Na+ and K+ permeability of K+ channel in plasma membrane, isolated from roots of the salt-tolerant mutant of wheat, was lower than that of wild type in 100 mmol/L KCl and NaCl solution. The opening frequency of K+ channel of the mutant reduced more significantly than that of wild type in two kinds of solution mentioned above. It is assumed that the reduction of opening frequency mainly contributes to the Na+ and K+ permeability of K+ channel of the mutant. The electric conductance of single-channel of the mutant was similar to that of wild type and the main difference between them was exhibited as the opening frequency. Their K+/Ka+ selectivity of K+ channel had no significant difference. The K+/Na+ selectivity of the mutant and wild type was 3.35 and 3.18 respectively.
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References
Greenway, H., Munns, H., Mechanism of salt tolerance in nonhalophytes,Annu. Rev. Plant Physiol., 1980, 31: 149.
Schrueder, J. I., Werd, J. M., Gasmann. W., Persprctives on the physiology and stmcture of inward-rectifying K+ channels in higher plants: biophysical implications for K+ uptake,Annu. Rev. Biophys. Biomol. Struct., 1994, 23: 441.
Rains, D. W., Epstein, E., Sodium absorption hv barley roots. Its mediation by mechanism 2 of alkali cation transport,Plant Physiol., 1967, 42: 319.
Schachtman, D. P., Schroeder, J. I., Structurc and transport mechanism of a high-affinity potassium uptake transporter from higher plants,Nature, 1994, 370: 655.
Anderson, J. A., Huprikar, S.S., Kochian, L. V.et al., Functional expression of a probableArabidopsis thaliana putassium channel inSacchuomyces cerevisae, inProc. Natl. Acad. Sci. USA, 1992, 89: 3736.
Schachtman, D. P., Tyerman, S. D., Teny, B. R., The K+/Na+ selectivity of a cation channel in the plasma membrane of mot cells does no differ in salt-tolerant and salt-sensitive wheat species,Plant Physiol., 1991, 97: 598.
Larsson, C., Widell, S., Kjellbm, P., Preparation of high-purity plasma membrane,Methods, Enzumol., 1987, 148: 558.
Li Legong, Yin Kuide, Yan Jiqionget al., Salt-adaptive mechanisms of K+ -channel in plasma membrane of tobacco callus,Chinece Science Bulletin, 1996, 41(13): 1123.
Wegner, L. H., De Boer, A. H., Two inward K+ channels in the xylem parenchyma cells of barley roots are regulated by G protein modulators through a membrane-delimited pathway,Planta. 1997, 203: 506.
Murata, Y., Obi, I., Ynshihashi, M.et al., Reduced permeability to K+ and Na+ ions of K+ channels in the plasme membrane of tobacco celln in suspension after adaptation to 50 mM NaCI,Plant Cell Physiol., 1994, 35(1): 87.
Niu, X., Bressan, R. A., Hasegawa, P. M.et al., Ion homeostasis in NaCl environments,Plant Phyaiol., 1995, 109: 735.
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Guo, F., Tang, Z. Reduced Na+ and K+ permeability of K+ channel in plasma membrane isolated from roots of salt-tolerant mutant of wheat. Chin. Sci. Bull. 44, 816–821 (1999). https://doi.org/10.1007/BF02885027
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DOI: https://doi.org/10.1007/BF02885027