Skip to main content
SpringerLink
Log in

Effect of internal deletion of Myosin II on growth and development ofDictyostelium discoideum

  • Notes
  • Published:
Chinese Science Bulletin

Abstract

Four deletion mutantDictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1 157–1454 (ΔS2-2), were transformed by standard electfoporation into mhcA-cells (T-null), a mutantDictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin II s under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affeds the growth and development ofDictyastelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. De Lozanne, A., Spudich, J. A., Disruption of theDictyostelium myosin heavy chain gene by homologous recombination,Science, 1987, 236: 1086.

    Article  PubMed  Google Scholar 

  2. Spudich, J. A., In pursuit of myosin function,Cell Regulation, 1989, 1:1.

    PubMed  CAS  Google Scholar 

  3. Sussman, M., Cultivation and synchronous morphogenesis ofDictyostelium under controlled experimental conditions,Meth. Cell Biol., 1987, 28: 9.

    Article  CAS  Google Scholar 

  4. Egelhoff, T. T., Brown, S. S., Spudich, J. A., Spatial and temporal control of nonmuscle myosin localization: Identification of a domain that is necessary for myosin filament disassemblyin vivo, J. Cell Biol., 1991, 112: 677.

    Article  PubMed  CAS  Google Scholar 

  5. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4,Nature, 1970, 227: 680.

    Article  PubMed  CAS  Google Scholar 

  6. Kubalek, E. W., Uyeda, T. Q. P., Spudich, J. A., ADictyostelium myosin II lacking a proximal 58-KDa portion of the tail is functionalin vitro andin vivo, Mol. Biol. Cell, 1992, 3: 1455.

    PubMed  CAS  Google Scholar 

  7. Uyeda, T. Q. P., Titus, M. A., The myosin ofDictyostelium, Dictyostelium-the model organism for basic biology, Tokyo: Tokyo University Press, 1997.

    Google Scholar 

  8. Harrington, W. F.et al., Contraction of myofibrils in the presence of antibodies to myosin subfragment 2, inProc. Natl. Acad. Sci. USA, 1990, 87: 7453.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Biological Sciences, China Agriculrural University, 100094, Beijing, China

    Shi Shu, Aixiao Liu, Guoqin Liu & Longfei Yen

  2. Bionic Design Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, 305-8562, Ibaraki, Japan

    Taro Q. P. Uyeda

Authors
  1. Shi Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Taro Q. P. Uyeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aixiao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guoqin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Longfei Yen
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Shu, S., Uyeda, T.Q.P., Liu, A. et al. Effect of internal deletion of Myosin II on growth and development ofDictyostelium discoideum . Chin. Sci. Bull. 44, 811–816 (1999). https://doi.org/10.1007/BF02885026

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02885026

Keywords

Search

Navigation