Skip to main content
Log in

Molecular cloning and expression analysis of a novel human cDNA fragment encoding a putative Ser/Thr protein kinase

  • Papers
  • Published:
Chinese Science Bulletin

Abstract

A novel full-length cDNA encoding a putative serine/threonine kinase has been isolated from a human testis cDNA library. A nucleotide sequence of 1225 bp length has been determined containing an open reading frame of 1 044 nucleotides (encoding 348 amino acids). In view of its degree of homology to members of the Ser/Thr protein kinase family and the closest relationship toMus musculus STK-1, the predicted product was designated by the name of HUMSTK-1. Its mRNA is present in large amounts in thymus, and small amounts in testis, small intestine and colon.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Hunter, T., Coopr, J. A.,The Enzymes, Vol. 17 (eds. Royer, P. D., Krebs, E. G.), Orlando: Academic Press, 1986, 191.

    Google Scholar 

  2. Edelman, A. M., Blumenthal, D. K., Krebs, E. G., Protein serirne/threonine kinases,Annu. Rev. Biochem., 1987, 56: 567.

    PubMed  CAS  Google Scholar 

  3. Hanks, S. K., Quinn, M. A., Hunter, T., The protein kinase family conserved features and deduced phylogeny of the catalytic. domains,Science, 1988, 241: 42.

    Article  PubMed  CAS  Google Scholar 

  4. Yarden, Y., Ullrich, A., Growth factor receptor tyrosine kinsase,Annu. Rev. Biochem., 1988, 57: 443.

    Article  PubMed  CAS  Google Scholar 

  5. Ihle, J. N., Cytokinr rrceptor signallng,Nature, 1995. 377: 591.

    Article  PubMed  CAS  Google Scholar 

  6. Kishida, E., Gotoh, Y., The MAP kirlase cascade is essential for diverse signal transduction pathways,Trends Biochem. Sci., 1993, 18: 128.

    Article  Google Scholar 

  7. Wernmaster, G., Pawson, T., A lysine in the ATP-binding site of pl30gag-fps is essential for protein-tyrosine kinase activity,J. Biol. Chem., 1986, 261: 328.

    Google Scholar 

  8. Hanks, S. K., Eukaryotic protein kinase,Curr. Opin. Struct. Biol., 1991, 4: 369.

    Article  Google Scholar 

  9. Niwa, H., Abe, K., Kunisada, T.et al., Cell-cycle-dependant expression of the STK-I gene encoding a novel murine putative protein kinase,Gene., 1996, 169(2): 197.

    Article  PubMed  CAS  Google Scholar 

  10. Kozak, M., Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNA,Nucleic Acids Res., 1984. 12: 857.

    Article  PubMed  CAS  Google Scholar 

  11. Altschul, S. F., Gish, W., Miller, W.et al., Basic local alignment searrh tool,J. Mol. Biol., 1990, 215: 403.

    PubMed  CAS  Google Scholar 

  12. Terada, Y., Tetsuka, M., Suzuki, F.et al., AIM-I: a mammalian midbody-associated protein required for cytokinesis,EMRO J., 1998, 17: 667.

    CAS  Google Scholar 

  13. Glover, D. M., Leibowitz, M. H., McLean, D. A.et al., Mutations in aurora prevrnt centresome separation leading to the formation of monopolar spindles,Cell. 1995. 81: 95.

    Article  PubMed  CAS  Google Scholar 

  14. Yanai, A., Arama, E., Kilfin, G.et al., ayk l, A movel mammalian gene related to Drosophila aurora centrosome separation kinase, is specificdlly expressed during meiosis,Oncogene. 1997, 14: 2943.

    Article  PubMed  CAS  Google Scholar 

  15. Kimura, M., Kotani, S., Hattori, T.et al., Cell cycle-depntlent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipll,J. Biol. Chem., 1997, 272(21): 13766.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

About this article

Cite this article

Zhang, Q., Dai, F., Zhang, M. et al. Molecular cloning and expression analysis of a novel human cDNA fragment encoding a putative Ser/Thr protein kinase. Chin. Sci. Bull. 44, 778–783 (1999). https://doi.org/10.1007/BF02885018

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02885018

Keywords

Navigation