Abstract
In order to study the molecular mechanisms of ELF MF biological effects, the differential display (DD) technology has been used to isolate differentially expressed cDNA fragments in Daudi cells after 50 Hz MF exposure and sham-exposure. Using total RNA isolated from 0.8 mT 50 Hz MF exposed cells and those from sham-exposed cells, one differentially regulated transcript has been found by DD and the expression pattern of the same transcript has been verified by reverse-Northern and Northern analyses. DNA sequence analysis and Genbank research demonstrated that the cDNA fragment (MF-CA) is a novel gene in human cell which is induced by ELF MF.
Similar content being viewed by others
References
Savitz, D., Overview of epidemiologic research on electric and magnetic fields and cancer, Am. Ind. Hyg. J., 1993, 54:197.
Taubes, G., EMF-cancer links: yes, no and maybe, Science, 1993, 262: 649.
Feychting, ML, Ahibom, A., Magnetic fields, leukemia and central-nervous-system tumors in Swedish adults residing near high-voltage power-lines, Epidemiology, 1994, 5: 501.
Wei, L. X., Goodman, R., Henderson, A., Changes in levels of c-myc and histone H2B following exposure of cells to low-frequency sinusoidal electromagnetic fields: Evidence for a window effect, Bioelectromagnetics, 1990, 11: 269.
Lacy-Hulbert, A., Roger, C., Wilkins, T. et al., Cancer risk and electromagentic fields, Nature, 1995, 375: 23.
Saffer, S. D., Thurston, S. J., Short exposure to 60 Hz magnetic fields do not altermyc expression in HL60 or Daudi cells, Radiation Res., 1995. 144:18.
Binninger, D. M., Ungvichian, V., Molecular cloning and characterization of 60 Hz EMF-responsive genes in the yeast, saccharomyces cerevisiae, in Abstract Book, Second World Congress for Electricity and Magnetism in Biology and Medicine, Bologna: The Bioelectromagnetics Society, USA, 1997, 241.
Liang, P., Pardee, A. B., Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction, Science, 1992, 257:967.
Liang, P., Pardee, A. B., Improvements to the differential display method for gene analysis, Biochemical and Biophysical Research Communications, 1994, 199: 564.
Sambrook, J., Maniatis, T., Fritsch, E. F., Molecular Cloning: A Laboratory Manual, 2nd ed. (translated by Jin, D. Y.), Beijing: Science Press, 1992, 372.
Dibirdik, I., Kristupaitis, D., Kurosaki, T. et al., Stimulation of Src family protein-tyrosine kinases as a proximal and mandatory step for SYK kinase-dependent phospholipase Cr2 activation in Lymphoma B cells exposed to low energy electromagnetic fields. J. Biol. Chem., 1998, 273: 4035.
Holmberg, B., Magnetic fields and cancer: Animal and cellular evidence-an overview, Environ. Health. Perspect, 1995, 103:63.
Wu, R. Y., Yang, H. J., Chiang, H. et al., The effects of low frequency magnetic fields on DNA unscheduled synthesis induced by methylnitro-nittrosoguanidinein vitro, Electro-and-magnetobiology, 1998, 17: 57.
Liburdy, R. P., Cellular interactions with electromagnetic fields: experimental evidence for field effects on signal transduction and cell proliferation, in On the Nature of Electromagnetic Field Interactions with Biological Systems (ed. Frey, A. H.), Austin: R. G. Lands Company, 1994, 99.
Loscher, W., Liburdy, R. P., Animal and cellular studies on carcinogenic effects of low frequency (50/60-Hz) magnetic fields, Mutation Res., 1998, 185.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Wu, R., Jiang, H., Hu, G. et al. Cloning and identification of magnetic field-responsive genes in Daudi cells. Chin. Sci. Bull. 45, 1006–1010 (2000). https://doi.org/10.1007/BF02884981
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02884981