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Expression of human hepatitis C virus core antigen in tobacco plants by tobacco mosaic virus-based vector system

Abstract

Tobacco mosaic virus (TMV) has the potential to highly express foreign gene. A novel TMV-basedin trans expression system was constructed. A TMV mutant TSHc had its coat protein replaced with hepatitis C virus (HCV) core antigen gene. Anotherr TMV mutant TSBD was replicase-defective. Coinfection of the two mutants could cause systemic infection in tobacco plants byin trans complementation of their functions. TSHc could effectively replicate and assemble to viral particles, which were a little longer than that of wild-type TMV. HCV core antigen was expressed in whole tobacco plants. A similar expression level of HCV core antigen was detected on serial passages, which suggested that this viral expression system be stable.

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Han, A., Liu, Y., Xiao, L. et al. Expression of human hepatitis C virus core antigen in tobacco plants by tobacco mosaic virus-based vector system. Chin. Sci. Bull. 45, 44–48 (2000). https://doi.org/10.1007/BF02884901

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  • DOI: https://doi.org/10.1007/BF02884901

Keywords

  • TMV vector
  • complementation
  • systemic infection
  • HCV core antigen