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Synthesis and energy transfer of model conjugate of phycobilisome rods

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Chinese Science Bulletin

Abstract

The conjugate of R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) was synthesized through a heterobifunctional coupling reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. The molar ratio of R-PE to C-PC was determined by absorption spectra, and the result was 2:1. The energy transfer phenomena were observed from steady-state fluorescence spectra. The calculated result showed that the energy transfer efficiency from R-PE to C-PC was 88%. The energy transfer kinetics was determined by picosecond time-resolved fluorescence spectra. The time constant of energy transfer from R-PE to C-PC was 80 ps, which was much longer than that in the rod of native phycobilisomes.

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References

  1. Gantt, E., Lipschultz, C. A., Energy transfer in phycobilisomes from phycoerythrin to allphycocyanin,Biochim. Biophys. Acta, 1973, 292: 858.

    Article  PubMed  CAS  Google Scholar 

  2. Glazer, A. N., Lundell, D. J., Yamanaka, G.et al., The structure of a simple phycobilisome,Annu. Microbial., 1983, 134B: 159.

    CAS  Google Scholar 

  3. Holzwarth, A. R., Structure-function relationships and energy transfer in phycobiliprotein antennae,Physiol. Plant., 1991, 83: 518.

    Article  CAS  Google Scholar 

  4. Gillbro, T., Sandstrom, V., Wendler, J.et al., Picosecond study of energy transfer kinetics in phycobilisomes ofSynechococcus 6301 and mutant AN112,Biochim. Biophys. Acta, 1985, 808: 52.

    Article  CAS  Google Scholar 

  5. Suter, G. W., Mazzola, P., Fluorescence decay kinetics in phycobilisomes from the bluegreen algaSynechococcus 6301,Biochim. Biophys. Acta, 1984, 766: 269.

    Article  CAS  Google Scholar 

  6. Piter, R. R., Edington, M. D., Beck, W. F., Protein-matrix solvation dynamics in the α subunit of C-phycocyanin,J. Phys. Chem., 1996, 100: 14198.

    Article  Google Scholar 

  7. Debreczeny, M. P., Sauer, K., Comparison of calculated and experimentally resolved rate constants for excitation energy transfer in C-phycocyanin. 1. Monomers,J. Phys. Chem., 1995, 99: 8412.

    Article  CAS  Google Scholar 

  8. Debreczeny, M. P., Sauer, K., Comparison of calculated and experimentally resolved rate constants for excitation energy transfer in C-phycocyanin. 2. Trimers,J. Phys. Chem., 1995, 99: 8420.

    Article  CAS  Google Scholar 

  9. Holzwarth, A. R., Wendler, J., Wehrmeyer, W., Picosecond time resolved energy transfer in isolated phycobilisomes fromRhodella violacea (Rhodophyceae), Photochem. Photobiol., 1982, 36: 479.

    Article  CAS  Google Scholar 

  10. Jiang Lijin, Ma Jinshi, He Huizhuet al., Some properties of R-phycoerythrin,Oceanologia et Limnologia Sinica (in Chinese), 1983, 14: 315.

    Google Scholar 

  11. Xia, A. D., Zhu, J. C., Jiang, L. J., Energy transfer kinetics in C-phycocyanin from cyanobacteriumWestiellopsis prolifica by pump-probe techniques,Biochem. Biophys. Res. Communs., 1991, 179: 558.

    Article  CAS  Google Scholar 

  12. Carlsson, J., Drevin, H., Axen, R., Proteinthiolation and reversible protein-protein conjugation: N-succinimidyl 3-(2-pyridyldithio) propionate, a new heterobifunctional reagent,Biochem. J., 1978, 173: 723.

    PubMed  CAS  Google Scholar 

  13. Yu Linghua, Zeng Fanjie, Jiang Lijinet al., Subunit composition and chromophore content of R-phycoerythrin fromPolysiphonia urceolata, Acta Biochimica et Biophysica Sinica, 1990, 22: 221.

    Google Scholar 

  14. MacColl, R., Guard-Friar, D.,Phycobiliproteins, Boca Raton: CRC Press, 1987, 64–67.

    Google Scholar 

  15. Zhao Jiquan, Zhao Jingquan, Yang Zixuanet al., The synthesis of phycobiliprotein cnjugates and their intramolecular energy transfer,Progress in Biochemistry and Biophysics (in Chinese), 1997, 24: 435.

    Google Scholar 

  16. Zhao Jingquan, Zhu Jinchang, Jiang Lijin, Study on the energy transfer processes in phycobilisomes from blue-green algae by the use of stochastic simulation approach,Biochim. Biophys. Acta, 1995, 1229: 39.

    Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Photographic Chemistry, Chinese Academy of Sciences, 100101, Beijing, China

    Jiquan Zhao, Jingquan Zhao & Lijin Jiang

  2. Institute of Laser and Spectra, Zhongshan University, 510275, Guangzhou, China

    Jingmin Zhang, Fuli Zhao, Xiguang Zheng & Hezhou Wang

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  1. Jiquan Zhao
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  2. Jingquan Zhao
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  3. Lijin Jiang
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  4. Jingmin Zhang
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  5. Fuli Zhao
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  6. Xiguang Zheng
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  7. Hezhou Wang
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Zhao, J., Zhao, J., Jiang, L. et al. Synthesis and energy transfer of model conjugate of phycobilisome rods. Chin. Sci. Bull. 44, 134–139 (1999). https://doi.org/10.1007/BF02884736

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  • DOI: https://doi.org/10.1007/BF02884736

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