Abstract
After repeated i.p. immunizations of mice with 10 μg of homopolymer poly (HPMA) no antibodies were detected by the ELISA test.
Immunization with copolymer P-Acap-Leu-HMDA leads to a weak antibody response, while immunization with a copolymer with someside chains modified with ARS or FITC groups (P-Acap-Leu-HMDA-ARS or P-Acap-Leu-HMDA-FITC) leads to a significant antibody response detectable by PFC, ELISA and haemagglutination tests. Most of these antibodies are aimed against the modifying haptenic group, a smaller amount against side oligopeptide sequences of the carrier.
Intensity of the antibody response depends on: 1) the antigen dose—the optimal dose was 10μg: both the higher (100μg) and the lower doses (1 and 0.1μg) induced considerably lower antibody responses; 2) molar mass of the immunizing fractions—fractions of high molar mass induced up to five times higher responses than those of a low molar mass; 3) the bound haptenic group—the ARS-copolymers induced ten times lower response than the FITC-copolymers.
We detected no difference between capacities of the H-2a, H-2b and H-2d haplotypes to react with anti-ARS antibodies after immunization with P-Acap-Leu-HMDA-ARS.
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Říhová, B., Ulbrich, K., Kopeček, J. et al. Immunogenicity of N-(2-hydroxypropyl)-methacrylamide copolymers—Potential hapten or drug carriers. Folia Microbiol 28, 217–227 (1983). https://doi.org/10.1007/BF02884085
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DOI: https://doi.org/10.1007/BF02884085