Abstract
As phospholipases ofSalmonella species may play a role in the pathogenesis of gastrointestinal tract diseases.Salmonella newport, the causative agent of infantile diarrhoea was examined for the production of phospholipase. The enzyme was purified by gel filtration chromatography and was found to be a protein of molar mass ranging from 43 to 67 kDa. The purified enzyme alone or in combination with organisms, produced both structural and functional changes in rabbit ileum, contributing towards pathogenesis of diarrhoea due to this organism.
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References
Bal P.P.: Studies on PLA ofSalmonella newport 27657.MSc Thesis. Panjab University, Chandigarh (India) 1983.
Balod S.B., Faris A., Krovacek K., Wadstrom T.: Enterotoxic and cytotoxic factors produced byS. enteritidis and S. typhimurium.Toxicon 21, 785 (1983).
Bhandari S., Asnani P.J.: Purification and characterization of phospholipase A ofS. oraeniberg.Ind.J.Comp.Microbiol.Immun.Inf.Dis. 10, 88–96 (1989).
Burton K.: Diphenylamine method for DNA estimation.Biochem.J. 62, 315–322 (1956).
Campbell D.H., Garvey S.J., Cremer, Susodorfd D.H.:Methods in Immunology. A Laboratory Test for Instruction and Research, 2nd ed. Benjamin, New York 1971.
Haberman E., Hardet K.L.: A sensitivie and specific plate test for quantitation of phospholipases.Ann.Biochem. 50, 163–173 (1972).
Hawk P.B.: Estimation of sodium and potassium in practical clinical biochemistry (H. Verley, Ed.), 4th ed., pp. 491–494. Arnold Heinemann Publishers (India) 1954.
Herbert D., Phipps P.J.: Orcinol method for RNA estimation.Methods Microbiol. 3, 289–290 (1971).
Kasai G.J., Burrows W.: The titration of cholera toxin and anti toxin in rabbit ileal loop.J.Inf.Dis. 116, 606 (1966).
Kaura Y.K., Sharma V.K., Chandiramani N.K.: Enterotoxigenicity and invasiveness ofSalmonella species.Antonie van Leeuwenhoek 48, 273–283 (1982).
Laemmli U.K., Favre M.: SDS-PAGE using modified Laemmli discontinuous buffer system: Maturation of the head of bacteriophage T4.J.Mol.Biol. 80, 575–599 (1973).
Miller G.L.: Use of dinitrosalicylic acid for determination of reducing sugars.Anal.Chem. 31, 426–428 (1959).
Neena: Studies on physico chemical and other properties of phospholipase a ofSalmonella newport.MSc Thesis. Panjab University, Chandigarh (India) 1987.
Peterson J.W.:Salmonella toxins, pp. 227–234 inPharmacology of Bacterial Toxins, Vol. 119 (F. Dorner, J. Drews, Eds). Pergamon Press, New York 1986.
Ronnberg B., Wadstrom J.: Rapid detection of heat labile enterotoxin in cell lysates from blood agar grown E. coli by a coagglutination test.J.Chem.Micr. 17, 1021–1024 (1983).
Schales D., Schales S.S.: Chloride estimation in microanalysis in medical biochemistry by Wooten, I.D.P. (1941).
Van Slyke: Cited fromStandard Methods in Clinical Chemistry 1, 19 (1953).
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Neena, Asnani, P.J. Structural and functional changes in rabbit ileum by purified extracellular phospholipase a ofSalmonella newport . Folia Microbiol 36, 572–577 (1991). https://doi.org/10.1007/BF02884039
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DOI: https://doi.org/10.1007/BF02884039