Abstract
A mutagenic effect of allylisothiocyanate (AITC) onE. coli WP 67 (trp uvrA polA) was only found in a test with metabolic activationin vitro. Mixed function oxidases containing cytochrome P-450 are not responsible for the conversion of AITC to mutagenic products. Liver microsomal fractions of rat, mouse, chinese hamster, goat and monkey were used forin vitro metabolic activation. The mutagenic effect of AITC differed in individual animal species according to the protein content in the microsomel fraction. Allylamine (formed by hydrolysis of AITC) did not exhibit any mutagenic effect.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Auerbach C., Robson J.M.: Production of mutations by allyl isothiocyanate.Nature 154, 81 (1944).
Bartsch H.: Predictive value of mutagenicity tests in chemical carcinogenesis.Mutation Res. 38, 177 (1976).
Bartsch H., Camus A., Malaveille C.: Comparative mutagenicity of N-nitrosamines in a semisolid and in a liquid incubation system in the presence of rat or human tissue fractions.Mutation Res. 37, 149 (1976).
Bridges B.A., Dennis R.D., Munson R.J.: Differential induction and repair of ultraviolet damage leading to true reversions and external suppressor mutations of an ochre codon inEscherichia coli B/r WP2.Genetics 57, 897 (1967).
Drobnica L.: The mechanism of the anti-yeast effect of isothiocyanates.Folia Microbiol. 9, 182 (1964).
Drobnica L.: Isothiocyanates as biologically active compounds. (In Slovak) DrSc Thesis. Chemical Faculty, Slovak Technical University, Bratislava (1970).
Drobnica L., Zemanová M., Nemec P., Antoš K., Kristián P., Štullerová A., Knoppová V., Nemec P., Jr.: Antifungal activity of isothiocyanates and related compounds. I. Naturally occurring isothiocyanates and their analogues.Appl. Microbiol. 15, 701 (1967).
Estabrook R.W., Franklin M.R., Cohen B., Shigamatzu A., Hildebrandt A.G.: Influence of hepatic microsomal mixed function oxidation reactions on cellular metabolic control.Metabolism 20, 187 (1971).
Fishbein L.:Chromatography of Environmental Hazards.Carcinogens, Mutagens and Teratogens. Vol. 1. Elsevier, Amsterdam 1972.
Fries N.: Viability and resistance of spontaneous mutations in Ophiostoma representing different degrees of heterotrophy.Physiol. Plantarum 1, 330 (1948).
Gielen J.E., van Cantfort J.: Organ selectivity and biochemical characteristics of aryl hydrocarbon hydroxylase (AHH) induction by cigarette smoke in rats and mice, p. 275 in R. Montesano, H. Bartsch, L. Tomatis (Eds.)Screening Tests in Chemical Carcinogenesis. Vol. 12. IARC Scientific Publications, Lyon 1976.
Green M.H.L., Muriel W.J.: Mutagen testing usingtrp + reversion inEscherichia coli.Mutation Res. 38, 3 (1976).
Guttenplan J.B., Hutterer F., Garro A.J.: Effects of cytochrome P-448 and P-450 inducers on microsomal dimethylnitrosamine demethylase activity and the capacity of isolated microsomes to activate dimethylnitrosamine to a mutagen.Mutation Res. 35, 415 (1975).
Hamajima F., Yasuhiro C., Nishihara S.: Action of allyl isothiocyanate, phenyl isothiocyanate and bithinol on glycolytic and oxidative metabolism in the larvae of nematode Anisakis species.Kiseichugaku Zasshi 18, 498 (1969).
Kacerovský O., Kalous J.: Blood flakes produced from freshly conserved blook by AITC in pig and poultry fodder. (In Czech)Biol. Chem. Animal Nutr. 8, 175 (1972).
Kawakishi S., Namiki M.: Decomposition of allyl isothiocyanate in aqueous solution.Agr. Biol. Chem. 33, 452 (1969).
Kojima M., Ogawa K.: Effects of isothiocyanates and their analogs on microorganism. II. Effects of decomposition products and related compounds of allyl isothiocyanate on the oxygen uptake of yeasts.Hakko Kogaku Zasshi 51, 260 (1973).
Kosker O., Esselen W.B., Fetters C.R.: Effect of allylisothiocyanate and related substances in the thermal resistance ofAspergillus niger, Saccharomyces ellipsoideus andBacillus thermoacidurans.Food Res. 16, 510 (1951).
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J.: Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265 (1951).
Major I.R.: Correlation of inicial changes in the mouse epidermal cell populations with two-stage carcinogenesis, a quantitative study.Brit. J. Cancer 24, 149 (1970).
Mohn G., Ellenberger J., Mc Gregor D., Merker H.J.: Mutations in plants, microorganisms and in host-mediated assay systems.Mutation Res. 29, 221 (1975).
Mudřík Z.: Pelleting of green fodder using AITC as conservation agent, p. 30 in:Collection of lectures at the seminar and Technological modifications of horned cattle fooder (In Czech). Centre of Scientific and Technical Information, Prague 1973.
Pazourková Z. Effect of allylisothiocyanate in nuclear division inPisum sativum.Biologia Plantarum 15, 44 (1973).
Rohrbach R., Hecker E., Sandritter W.: Cytophotometric determinations of DNA content in epidermis after unspecific cocarcinogenic and carcinogenic irritation.Z. Krebsforsch. 70, 211 (1968).
Říhová E.: Correction method for estimating the number of reversions in bacteria.Folia Microbiol. 26, 426 (1981).
Šrám R.J., Kulešov N.P.: Monitoring of genetic effects of environmental factors in the human population. (In Czech)Biological Papers 44, 81 (1979).
Teubner H., Weuffen W., Hoeppe H.: Relations between chemical constitution and antimicrobial action. Preparation of diisothiocyanates and their antimicrobial and antihelmintic activity.Arch. Exp. Veterinaermed. 29, 621 (1975).
Zahradníček J., Hašek J.: Chemical preservation of the fibrous tissue of the sugarbeet. (In Czech)Listy cukrov. 91, 21 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Říhová, E. Mutagenic effects of allylisothiocyanate inEscherichia coli WP 67. Folia Microbiol 27, 25–31 (1982). https://doi.org/10.1007/BF02883833
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02883833