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Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase Cζ

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Chinese Science Bulletin

Abstract

Protein kinase C (PKC), which has a wide variety of functions, comprises at least twelve isoenzymes that have distinct roles respectively. Research in recent years indicates that PKCζ plays a pivotal role in the regulation of cell proliferation. In order to investigate its role in the regulation of keratinocyte proliferation, eukaryotic expression plasmid of anti-sense PKCζ is constructed and transfected into human keratinocyte line, Colo16. The human keratinocyte model expressing anti-sense PKCζ is established successfully, the growth properties of the cell model is analyzed by the detection of cell growth curve and cell cycle phase. The mechanism of the relationship between PKCζ and CyclinD1 is also investigated by the western blotting analysis of CyclinDl protein.

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Author notes

  1. Xiaoyan Zhang

    Present address: Department of Dermatology of China-Japan Friendship Hospital in Beijing, 100029, Beijing, China

Authors and Affiliations

  1. State Key Laboratory of Cell Proliferation and Regulation Biology, Department of Biology, Beijing Normal University, 100875, Beijing, China

    Xiaoyan Zhang & Huitu Liu

  2. Department of Dermatology, The First Hospital, Beijing Medical University, 100034, Beijing, China

    Shengqing Ma

Authors
  1. Xiaoyan Zhang
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  2. Huitu Liu
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  3. Shengqing Ma
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Zhang, X., Liu, H. & Ma, S. Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase Cζ. Chin. Sci. Bull. 43, 1556–1562 (1998). https://doi.org/10.1007/BF02883448

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  • DOI: https://doi.org/10.1007/BF02883448

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