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Regulation of human chorionic gonadotropin secretion and messenger ribonucleic acid levels by follistatin in the NUCC-3 choriocarcinoma cell line

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Chinese Science Bulletin

Abstract

NUCC-3 choriocarcinoma cell line was used as anin vitro placental cell model to investigate the effects of follistatin on GnRH-stimulated hCG secretion and its subunit mRNA level and stability. Follistatin alone did not affect basal hCG secretion and its subunit mRNA level. GnRH increased hCG secretion and hCG β-mRNA level in a dose-dependent manner. Follistatin significantly suppressed GnRH-stimulated hCG secretion and hCG α- and β-mRNA level. It inhibited hCG secretion in response to PMA, and did not affect the stability of hCG α- and β-mRNA. The authors suggest that follistatin inhibits GnRH-stimulated hCG secretion as well as hCG α- and β-RNA level by decreasing the rate of transcription through the second messenger transduction system —protein kinase-C.

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References

  1. Ueno, N., Ling, N., Ying, S. Y.et al., Isolation and partial characterization of follistatin: a single-chain monomeric protein that inhibits the release of FSH,Proc. Natl. Acad. Sci. USA, 1987, 84: 8282.

    Article  PubMed  CAS  Google Scholar 

  2. Wang, Q. F., Farnworth, P. G., Burger, H. G.et al., Acute inhibitory effect of FSH-suppressing activity of follistatin and inhibin in ovariectomized rats,Mol. Cell Endocrinol., 1990, 72: 33.

    Article  PubMed  CAS  Google Scholar 

  3. Chomcynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,Anal. Biochem., 1987, 162: 156.

    Google Scholar 

  4. Sugiura, M., Goto, S., Saito, M.et al., Establishment and characterization of three new human gestational choriocarcinoma cell line,Acta Obstet. Gynecol. Jpn., 1988, 40: 1447.

    CAS  Google Scholar 

  5. Siler-Khodr, T. M., Hypothalamic-like peptides of the placenta,Semin. Rep. Endocrinol., 1983, 1: 321.

    Article  Google Scholar 

  6. Belisle, S., Guevin, J. F., Bellabarba, D.et al., LHRH binds to enriched human placental membranes and stimulatesin vitro synthesis of bioactive hCG,J. Clin. Endocrinol. Metab., 1984, 59: 119.

    PubMed  CAS  Google Scholar 

  7. Siler-Khodr, T. M., Khodr, G. S., Vickery, B. H.et al., Inhibition of hCG, a hCG and progesterone release from human placental tissuein vitro by a GnRH antagonist,Life Sci., 1983, 32: 2741.

    Article  PubMed  CAS  Google Scholar 

  8. Posillico, E. G., Handwerger, S., Tyrey, L., HCG a subunit of normal placenta: characterization of synthesis and association with β subunit,Biol. Reprod., 1985, 32: 1101.

    Article  PubMed  CAS  Google Scholar 

  9. Redway, M., Zhou, F., Benoit, J.et al., Differential effects of 8-bromo-cyclic AMP on hCG, progesterone and estrogen production by term placental cells,Life Sci., 1988, 43: 1451.

    Article  Google Scholar 

  10. Nulsen, J. C., Woolkalis, M. J., Kope, G. S.et al., Adenylate cyclase in human cytotrophoblasts: characterization and its role in modulating hCG secretion.J. Clin. Endocrinol. Metab., 1988, 66: 258.

    PubMed  CAS  Google Scholar 

  11. Benoit, J., Rodway, M., Yuen, B. H.et al., Effects of cyclic adenosine monophosphate on hCG and estradiol output by cultured human placental cells,Am. J. Obstet. Gynecol., 1988, 158: 328.

    PubMed  CAS  Google Scholar 

  12. Fuchiwaki, T., Role of c-kinase in development and function of human placental chorionic tissue,Acta Obstet. Gynecol. Jpn., 1987, 120: 1521.

    Google Scholar 

  13. Conn, P. M., McArdle, C. A., Andrews, W. V.et al., The molecular basis of GnRH action in the pituitary gonadotrope,Biol. Reprod., 1987, 36: 17.

    Article  PubMed  CAS  Google Scholar 

  14. Nishino, R., Maruo, T., Matsuo, H.et al., The role of signal transduction systems in the regulation of the production and secretion of hCG (α, β) by cultured human choriocarcinoma cells (BeWo),Nippon Naibunpi Gakkai Zasshi, 1991, 67: 666.

    PubMed  CAS  Google Scholar 

  15. Peak, I., Axel, R., Glucocorticoids enhance stability of human growth hormone mRNA,Mol. Cell Biol., 1987, 7: 1496.

    Google Scholar 

  16. Peterson, D. D., Koch, S. R., Granner, D. K., 3′ noncoding region of phosphoenolpyuvate carboxykinase mRNA contains a glucocorticoid-response mRNA-stabilizing element,Proc. Natl. Acad. Sci. USA, 1989, 86: 7800.

    Article  Google Scholar 

  17. Carroll, R. S., Corrigan, A. Z., Vale, W.et al., Activin stabilizes FSH β mRNA level,Endocrinology, 1991, 129: 1721.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Beijing Medical University, 100083, Beijing, China

    Lingyun Shi, Hongbo Li & Zhiwen Zhang

  2. National Research Institute of Sports Medicine, 100029, Beijing, China

    Zilong Fang

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  1. Lingyun Shi
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  2. Zilong Fang
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  3. Hongbo Li
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  4. Zhiwen Zhang
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Shi, L., Fang, Z., Li, H. et al. Regulation of human chorionic gonadotropin secretion and messenger ribonucleic acid levels by follistatin in the NUCC-3 choriocarcinoma cell line. Chin. Sci. Bull. 43, 1635–1642 (1998). https://doi.org/10.1007/BF02883409

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  • DOI: https://doi.org/10.1007/BF02883409

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