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Nuclear matrix of the most primitive eukaryoteArchezoa

Science in China Series C: Life Sciences volume 41, pages 479–487 (1998)Cite this article

Abstract

Accumulating evidence suggests that unicellularArchezoa are the most primitive eukaryotes and their nuclei are of significance to the study of evolution of the eukaryotic nucleus. Nuclear matrix is an ubiquitous important structure of eukaryotic nucleus; its evolution is certainly one of the most important parts of the evolution of nucleus. To study the evolution of nuclear matrix, nuclear matrices ofArchezoa are investigated.Giardia lamblia cells are extracted sequentially. Both embedment-free section EM and whole mount cell EM of the extracted cells show that, like higher eukaryotes, this species has a residual nuclear matrix in its nucleus and rich intermediate filaments in its cytoplasm, and the two networks connect with each other to form a united network. But its nuclear matrix does not have nucleolar matrix and its lamina is not as typical as that of higher eukaryotes; Western blotting shows that lamina ofGiardia and two otherArchezoa Entanzoeba invadens andTrichomonas vaginali all contain only one polypeptide each which reacts with a mammalia anti-lamin polyclonal serum and is similar to lamin B (67 ku) of rnammiia in molecular weight. According to the results and references, it is suggested that nuclear matrix is an early acquisition of the eukaryotic nucleus, and it and the “eukaryotic chromatin” as a whole must have originated very early in the process of evolution of eukaryotic cell, and their origin should be an important prerequisite of the origin of eukaryotic nucleus: in the lamin (gene) family, B-type lamins (gene) should be the ancestral typz and that A-type lamins (gene) might derive therefrom.

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Affiliations

  1. Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223, Kunming, China

    Jianfan Wen & Jingyan Li

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  1. Jianfan Wen
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  2. Jingyan Li
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Project supported by the National Natural Science Foundation of China (Grant No. 3870254).

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Wen, J., Li, J. Nuclear matrix of the most primitive eukaryoteArchezoa . Sci. China Ser. C.-Life Sci. 41, 479–487 (1998). https://doi.org/10.1007/BF02882885

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  • DOI: https://doi.org/10.1007/BF02882885

Keywords

  • Archezoa
  • nuclear matrix
  • nuclear lamina
  • origin and evolution