Abstract
A klnd of cytotoxinic gene barnase was cloned downstream of Immediate early promoter from cytomegalovirus (CMV). Rerombinant virus vAcCMVBar was constructed uslng Bac-to-Bac system by insertion of the expresslon cassette Into the polyhedrin gene locus ofAutographa californtca nuclear polyhedrosis virus (AcNPV). Cytotoxinic effect caused by expression of barnase was observed in four mammalian cells after vAcCMVBar infection Dose-dependent analysis revealed that one of the cells HICAM was the most sensitive to the virus infection. It was. shown that AcNPV could be used as gene transfer vector in mammalian cells and that barnase is the suitable report gene which could be detected more easily than that commonly used.
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Zhu, Y., Qi, Y., Liu, Z. et al. Baculovirus-mediated gene transfer into mammalian cells. Sci. China Ser. C.-Life Sci. 41, 473–478 (1998). https://doi.org/10.1007/BF02882884
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DOI: https://doi.org/10.1007/BF02882884