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Correlation between expression of cadherin and gap junctional communication in human lung carcinoma cells

Science in China Series C: Life Sciences volume 41, pages 419–425 (1998)Cite this article

Abstract

The correlation between expression of ca2+-dependent cell adhesion molecule, cadherin, and gap junctional intercellular communication (GJIC) in human lung carcinoma PG cell line and connexin43 (Cx43) cDNA transfected PG cell clones was investigated. Results from immunoblotting and immunofluorescent staining revealed that cultured normal human lung cells (RF) expressed N-cadherin. However, the expression level of Kcadherin in PG cells was very low in comparison with normal RF cells. The Cx43 transfected PG clones exhibited comparable levels of Cx43 protein, but varied in the level of N-cadherin expression and in the function of GJIC as measured by scrape-loading and dye transfer (SLDT) method. Positive correlation between N-cadherin and GJIC was demonstrated. Thein vitro andin uivo, growth examination results suggest that N-cadherin mediated cell-cell adhesions and Cx43 functional expression, the GJIC, may work coordinately with each other in regulation of cell growth and differentiation. Deficiency in both GJIC and cell adhesion may be crucial for cell transformation.

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References

  1. Hatta, K., Takagi, S., Fujisawa, H. etal., Spatial and temporal expression pattern of N-cadherin cell adhesion molecules correlated with morphogenetic processes of chicken embryos,Developmental Biology, 1987, 120:215.

    PubMed  Article  CAS  Google Scholar 

  2. Huber, O., Bierkamp, C., Kernler, R. et al., Cadherins and catenins in development,Current Opinion in Cell Biology, 1996, 8: 685.

    PubMed  Article  CAS  Google Scholar 

  3. Gumbiner, B. M., Cell adhesion: the molecular basis of tissue architecture and morphogenesis,Cell, 1996, 84: 345.

    PubMed  Article  CAS  Google Scholar 

  4. Bruzzune, R., White, T. W., Goodenough, D. A., The cellular internet: on-line with connexins,BioEssays, 1996, 18: 709.

    Article  Google Scholar 

  5. Meyer, R. A., Laird, D. W., Revel, J. P. etal., Inhibition of gap junction and adherents junction assembly by connexin and A-CAM antibodies,J. Cell Biol., 1992, 119: 197.

    Google Scholar 

  6. Guger, K. A., Gumbiner, B. M., β-catenin has Wnt-like activity mimics the nieuwkoop signaling center in Xenopus dorsalventral patterning,Developmental Biology, 1995, 172: 115.

    PubMed  Article  CAS  Google Scholar 

  7. Zhang, Z. Q., Lin, Z. X., Han, Y. L. etal., Expression of gap junction protein Cx43 in cultured normal human embryonic lung cells and lung carcinoma cells,Acta Biophysics Sinica (in Chinese), 1994, 4: 411.

    Google Scholar 

  8. Zhang, Z. Q., Lin, Z. X., Wang, N. Q. et al., Expression of gap junction and inhibition of cell growth in communicationdefected cells after transfection with gap junction gene Cx43 cDNA,Chinese Science Bulletin, 1996, 41: 1501.

    Google Scholar 

  9. Moorby, C. D., Stoker, M., Gherardi, E., HGF/SF inhibits junctional communication,Experimental Cell Research, 1995, 219: 657.

    PubMed  Article  CAS  Google Scholar 

  10. Nicolson, G. L., Dulski, K. M., Trosko, J. E., Loss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cells,Proc. Natl. Acad. Sci. USA, 1988, 85: 473.

    PubMed  Article  CAS  Google Scholar 

  11. Musil, L. S., Goodenough, D. A., Biochemical analysis of cornexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques,J. Cell Biol., 1991, 115: 1357.

    PubMed  Article  CAS  Google Scholar 

  12. Jongen, W. M. F., Fitzgerald, D. J., Asamoto, M. et al., Regulation of connexin43-mediated gap junctional intercellular communication by ca2+ in mouse epidermal cells is controlled by E-Cadherin,J. Cell Biol., 1991, 114: 545.

    PubMed  Article  CAS  Google Scholar 

  13. Gumbiner, B. M., Proteins associated with cytoplasmic surface of adhesion molecule,Neuron, 1993, 11: 551.

    PubMed  Article  CAS  Google Scholar 

  14. Paul, D. L., New functions for gap junction,Current Opinion in Cell Biology, 1995, 7: 665.

    PubMed  Article  CAS  Google Scholar 

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Affiliations

  1. Department of Cell Biology, Beijing Institute for Cancer Research, Beijing Medical University, School of Oncology, 100034, Beijing, China

    Wenjun Zhang, Zhiqian Zhang, Zhongxiang Lin, Naiqin Wang & Ying Hu

Authors
  1. Wenjun Zhang
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  2. Zhiqian Zhang
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  3. Zhongxiang Lin
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  4. Naiqin Wang
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  5. Ying Hu
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Project supported by the National Natural Science Foundation of China (Grant Nos. 39580005 and 397703801, Beijing Natural Science Foundation and Beijing New Star Program.

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Zhang, W., Zhang, Z., Lin, Z. et al. Correlation between expression of cadherin and gap junctional communication in human lung carcinoma cells. Sci. China Ser. C.-Life Sci. 41, 419–425 (1998). https://doi.org/10.1007/BF02882743

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  • DOI: https://doi.org/10.1007/BF02882743

Keywords

  • N-cadherin
  • Cx43
  • cell adhesion and communication
  • hwnan lung carcinoma cells
  • reverse transformation