Abstract
Using genomicin-situ hybridization (GISH) technique, 7 translocation-addition lines, 6 translocation and translocation-addition lines, 2 ditelosomic addition lines and 1 translocation line were identified fromTriticum aestivum L. -Psathyrostachys juncea (Fisch.) Nevski intergeneric hybrids, of which translocation-addition and translocation and translocation-addition lines were not found in other reports. No substitutions and disornic additions were detected in the, hybrids and breakages occurred in allP. juncea chromosomes studied. Results have shown that the improved GISH technique is a rapid and economical method for use in this field.
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References
Heslop-Harrison, J. S., Schwarzacher, T., Leitch, A.R.et al., A method of identifying DNA sequences in chromosomes of plants,European Patent Application Number 8828130. 8, 1988, December 2.
Le, H. T., Armstrong, K. C., Miki, B., Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe,Plant Mol. Biol. Reporter, 1989, 7: 150.
Reader, S. M., Abbo, S.. Purdie, K. A.et al., Direct labelling of plant chromosomes by rapidin situ hybridization,Trend Genet., 1994, 10:265.
Schwarzacher, T., Leitch, A. R., Bennett, M. D.et el., In situ localization of parental genomes in a wide hybrid,Ann. Bot., 1989, 64:315.
Schwarzacher, T., Heslop-Harrison, J.S., Anamthawat-Jonsson, K.et al., Parental genome separation in reconstmctions of somatic and premeiotic metaphases ofHordeum vulgare X H.bulbosum, J. Cell Sci., 1992, 101:13.
Leitch, A.R., Mosgoller, W., Schwarzacher, T.et al., Genomicin-situ hybridization to sectioned nuclei shows chmmosome domains in grass hybrids,J. Cell Sci., 1990, 95:335.
Anamthawat-Jonsson, K., Heslop-Harrison, J. S.et al., Isolation and characterization of genome-specific DNA sequence inTriticeae species,Mol. Gen. Genet., 1993, 240:151.
Schwarzacher, T., Anamthawat-Jónsson, K., Harrison, G.E.et al., Genomicin situ hybridization to identify alien chromosomes and chromosome segment in wheat,Theor. Appl. Genet., 1992, 84:778.
Chen Qin, Zhou Ronghua, Li Lihuiet al., First intergeneric hybrid betweenTriticum aestivum andPsathyrostachys juncea, Kexue Tongbao, 1988, 33(24): 2071.
Leitch, I.J., Heslop-Harrison, J.S., Detection of digoxigenin-labeled DNA probes hybridized to plant chromosomesin situ, in Methods in Molecular Biology, Vol.28:Protocols for Nucleic Acid Analysis by Nonradiwctive Probes, Humana, Totowa, NJ, 1994, 177–185.
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Zhou, R., Jia, J., Dong, Y. et al. Characterization of progenies ofTriticum aestivum-Psathyrostachys juncea derivatives by using genomicin-situ hybridization. Sci. China Ser. C.-Life Sci. 40, 657–664 (1997). https://doi.org/10.1007/BF02882697
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DOI: https://doi.org/10.1007/BF02882697