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Relation between nuclear envelope and nuclear lamina in nuclear assemblyin vitro

Science in China Series C: Life Sciences volume 40, pages 576–582 (1997)Cite this article

Abstract

Xenopus laevis egg extracts cell-free nuclear assembly system was used as an experimental model to study the process of nuclear lamina assembly in nuclear reconstitutionin vitro. The experimental results showed that lamin was involved in the nuclear assemblyin vitro. The assembly of nuclear lamina was preceded by the assembly of nuclear matrix, and probably, inner nuclear matrix assembly provided the basis for nuclear lamina assembly. Inhibition of normal assembly of nuclear Iknina, by preincubating egg extracts cell-free system with anti-lamin antibodies, resulted in abnormal assembly of nuclear envelope, suggesting that nuclear envelope assembly is closely associated with nuclear lamina assembly.

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References

  1. Lohka, M., Masui, Y., Formationin vitro of sperm pronuclei and mitotic chromosome induced by amphibian ooplasmic components,Science, 1983, 220(4598): 719.

    PubMed  Article  CAS  Google Scholar 

  2. Newport, J., Nuclear reconstitutionin vitro: stages of assembly around protein-free DNA,Cell, 1987, 4812): 205.

    Article  Google Scholar 

  3. Cai, S., Zhai, Z., Nuclear lamina-an important nuclear structure,Chinese Journal of Cell Biology (in Chinese), 1990, 12(1): 15.

    Google Scholar 

  4. Gerace, L., Nuclear lamina and organization of nuclear architecture,TIBS, 1986, 11(11): 443.

    CAS  Google Scholar 

  5. Gerace, L., Blobel, G., The nuclear envelope lamina is reversibly depolymerized during mitosis,Cell, 1980, 19(2): 277.

    PubMed  Article  CAS  Google Scholar 

  6. Jost, E., Johnson, J. T., Nuclear lamina assembly, synthesis and disaggregation during the cell cycle in synchronized cells,J. Cell Sci., 1981, 47(1): 25.

    PubMed  CAS  Google Scholar 

  7. Burke, B., Gerace, L., A cell-free system to study reassembly of the nuclear envelope at the end of mitosis,Cell, 1986, 44(4): 639.

    PubMed  Article  CAS  Google Scholar 

  8. Cai, S., Zhang, B., Zhang, C.et al., Nuclear reconstitution inXenopus laevis egg extracts cell-free system,Chinese Science Bulletin (in Chinese), 1990, 32(11): 1261.

    Google Scholar 

  9. Zhang, B., Zhai, Z., Ultrastructural study on cell-free nuclear reconstitution,Acta Anatomia Sin. (in Chinese), 1992, 22(1): 48.

    Google Scholar 

  10. Fey, E.G., Krochmalnic, G., Penman, S., The nonchromatin matrix of nucleus: protein and RNA components revealed by sequential fractionation and resinless TEM section,J. Cell Biol., 1986, 102(5): 1645.

    Article  Google Scholar 

  11. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4,Nature, 1970, 227(5259): 680.

    PubMed  Article  CAS  Google Scholar 

  12. Towbin, H., Staehelin, T., Gordon, T., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications,Proc. Natl. Acad. Sci. USA, 1979, 76(9): 4350.

    PubMed  Article  CAS  Google Scholar 

  13. Cai, S., Zhai, Z., Nuclear matrix in the nuclei assembledin vitro, Chinese Science Bulletin (in Chinese), 1993, 34(13): 1223.

    Google Scholar 

  14. Benavente, R., Krohne, G., Involvement of nuclear lamins in postmitotic reorganization of chromatin demonstrated by micminjection of lamin antibodies,J. Cell Biol., 1986, 103(6): 1847.

    PubMed  Article  CAS  Google Scholar 

  15. Dabauvalle, M. C., Loss, K., Merkert, H.et al., Spontaneous assembly of pore complex-containing membranes (“annulate lamellae”) inXenopus egg extracts in the absence of chromatin,J. Cell Biol., 1991, 112(6): 1073.

    PubMed  Article  CAS  Google Scholar 

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Author notes

  1. Shutao Cai

    Present address: Institute of Biophysics, The Chinese Academy of Sciences, 100101, Beijing, China

Affiliations

  1. Life Sciences College of Peking University, 100871, Beijing, China

    Shutao Cai & Zhonghe Zhai

Authors
  1. Shutao Cai
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  2. Zhonghe Zhai
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Project supported by the National Natural Science Foundation of China.

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Cai, S., Zhai, Z. Relation between nuclear envelope and nuclear lamina in nuclear assemblyin vitro . Sci. China Ser. C.-Life Sci. 40, 576–582 (1997). https://doi.org/10.1007/BF02882687

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  • DOI: https://doi.org/10.1007/BF02882687

Keywords

  • nuclear assemblyin vitro
  • nuclear envelope
  • nuclear lamina
  • nuclear matrix