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Isolation of development-related genes in somatic embryo radicle of carrot (Daucus carota L.) using modified cDNA RDA

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Abstract

Using modified cDNA RDA capitalizing on the high affinity of streptavidin for biotin and magnetic-absorption-based separation, we have obtained four bands of specifically expressed cDNA in the carrot somatic embryo deregulated for 12 h, which were designated as NR-1, NR-2, NR-3 and NR-4, respectively. As revealed by homology analysis of their DNA sequences after cloning them into pBS, remarkable homology was demonstrated in NR-2, NR-3 and NR-4 with the genes coding for LEA (late embryogenesis abundant protein), Dna J and xyloglucan endo-transglycosylase in plants. On the other hand, NR-1 showing no homology with any known sequence may have come from unknown genes. Using32P-labeled NR-1 as probe, hybridization with cDNA fragment population has shown that we have actually cloned a new gene fragment related to radicle development. As shown by further Southern hybridization, these genes may be present in carrot genome in the form of single or low copies.

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Authors and Affiliations

  1. Institute of Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China

    Fengqiu Diao, Lei Zhang & Naihu Wu

  2. College of Life Sciences, Peking University, 100871, Beijing, China

    Meijuan Huang

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  1. Fengqiu Diao
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  2. Lei Zhang
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  3. Meijuan Huang
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  4. Naihu Wu
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Correspondence to Naihu Wu.

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Diao, F., Zhang, L., Huang, M. et al. Isolation of development-related genes in somatic embryo radicle of carrot (Daucus carota L.) using modified cDNA RDA. Sci. China Ser. C.-Life Sci. 43, 47–56 (2000). https://doi.org/10.1007/BF02881717

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  • DOI: https://doi.org/10.1007/BF02881717

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